Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

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SHR Neuro Krebs Kardio Lipid

Depaoli, MR; Karsten, F; Madreiter-Sokolowski, CT; Klec, C; Gottschalk, B; Bischof, H; Eroglu, E; Waldeck-Weiermair, M; Simmen, T; Graier, WF; Malli, R.
Real-Time Imaging of Mitochondrial ATP Dynamics Reveals the Metabolic Setting of Single Cells.
Cell Rep. 2018; 25(2):501-512 [OPEN ACCESS]
Web of Science PubMed FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Bischof Helmut
Depaoli Maria Rosa
Eroglu Emrah
Gottschalk Benjamin
Graier Wolfgang
Karsten Felix Daniel
Klec Christiane
Madreiter-Sokolowski Corina
Malli Roland
Waldeck-Weiermair Markus
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Abstract:
Reprogramming of metabolic pathways determines cell functions and fate. In our work, we have used organelle-targeted ATP biosensors to evaluate cellular metabolic settings with high resolution in real time. Our data indicate that mitochondria dynamically supply ATP for glucose phosphorylation in a variety of cancer cell types. This hexokinase-dependent process seems to be reversed upon the removal of glucose or other hexose sugars. Our data further verify that mitochondria in cancer cells have increased ATP consumption. Similar subcellular ATP fluxes occurred in young mouse embryonic fibroblasts (MEFs). However, pancreatic beta cells, senescent MEFs, and MEFs lacking mitofusin 2 displayed completely different mitochondrial ATP dynamics, indicative of increased oxidative phosphorylation. Our findings add perspective to the variability of the cellular bioenergetics and demonstrate that live cell imaging of mitochondrial ATP dynamics is a powerful tool to evaluate metabolic flexibility and heterogeneity at a single-cell level. Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

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