Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

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Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid

Schilcher, I; Kern, S; Hrzenjak, A; Eichmann, TO; Stojakovic, T; Scharnagl, H; Duta-Mare, M; Kratky, D; Marsche, G; Frank, S.
Impact of Endothelial Lipase on Cholesterol Efflux Capacity of Serum and High-density Lipoprotein.
Sci Rep. 2017; 7(1): 12485-12485. [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG


Autor/innen der Med Uni Graz:
Duta-Mare Madalina-Cristina
Frank Saša
Hrzenjak Andelko
Kratky Dagmar
Marsche Gunther
Scharnagl Hubert
Schilcher Irene Rosa Maria
Stojakovic Tatjana

Dimensions Citations:

Plum Analytics:
Number of Figures: 6
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Endothelial lipase (EL) is a potent modulator of the structural and functional properties of HDL. Impact of EL on cholesterol efflux capacity (CEC) of serum and isolated HDL is not well understood and apparently contradictory data were published. Here, we systematically examined the impact of EL on composition and CEC of serum and isolated HDL, in vitro and in vivo, using EL-overexpressing cells and EL-overexpressing mice. CEC was examined in a validated assay using (3)H-cholesterol labelled J774 macrophages. In vitro EL-modification of serum resulted in complex alterations, including enrichment of serum with lipid-free/-poor apoA-I, decreased size of human (but not mouse) HDL and altered HDL lipid composition. EL-modification of serum increased CEC, in line with increased lipid-free/-poor apoA-I formation. In contrast, CEC of isolated HDL was decreased likely through altered lipid composition. In contrast to in vitro results, EL-overexpression in mice markedly decreased HDL-cholesterol and apolipoprotein A-I serum levels associated with a decreased CEC of serum. HDL lipid composition was altered, but HDL particle size and CEC were not affected. Our study highlights the multiple and complex effects of EL on HDL composition and function and may help to clarify the seemingly contradictory data found in published articles.

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