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Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid

Schlager, S; Vujic, N; Korbelius, M; Duta-Mare, M; Dorow, J; Leopold, C; Rainer, S; Wegscheider, M; Reicher, H; Ceglarek, U; Sattler, W; Radovic, B; Kratky, D.
Lysosomal lipid hydrolysis provides substrates for lipid mediator synthesis in murine macrophages.
Oncotarget. 2017; 8(25):40037-40051 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG


Autor/innen der Med Uni Graz:
Duta-Mare Madalina-Cristina
Korbelius Melanie
Kratky Dagmar
Leopold Christina
Radovic Branislav
Rainer Silvia
Reicher Helga
Sattler Wolfgang
Schlager Stefanie
Vujic Nemanja
Wegscheider Martin

Dimensions Citations:

Plum Analytics:
Number of Figures: 9
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Degradation of lysosomal lipids requires lysosomal acid lipase (LAL), the only intracellular lipase known to be active at acidic pH. We found LAL to be expressed in murine immune cells with highest mRNA expression in macrophages and neutrophils. Furthermore, we observed that loss of LAL in mice caused lipid accumulation in white blood cells in the peripheral circulation, which increased in response to an acute inflammatory stimulus. Lal-deficient (-/-) macrophages accumulate neutral lipids, mainly cholesteryl esters, within lysosomes. The cholesteryl ester fraction is particularly enriched in the PUFAs 18:2 and 20:4, important precursor molecules for lipid mediator synthesis. To investigate whether loss of LAL activity affects the generation of lipid mediators and to eliminate potential systemic effects from other cells and tissues involved in the pronounced phenotype of Lal-/- mice, we treated macrophages from Wt mice with the LAL-specific inhibitor LAListat-2. Acute inhibition of LAL resulted in reduced release of 18:2- and 20:4-derived mediators from macrophages, indicating that lipid hydrolysis by LAL is an important source for lipid mediator synthesis in macrophages. We conclude that lysosomes should be considered as organelles that provide precursor molecules for lipid mediators such as eicosanoids.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Carbamates - pharmacology
Cholesterol Esters - metabolism
Eicosanoids - metabolism
Female -
Hydrolysis -
Lipid Metabolism -
Lipids - analysis
Lipids - blood
Lysosomes - metabolism
Macrophages - drug effects
Macrophages - metabolism
Male -
Mice, Inbred C57BL -
Mice, Knockout -
Sterol Esterase - antagonists & inhibitors
Sterol Esterase - genetics
Sterol Esterase - metabolism
Substrate Specificity -
Thiadiazoles - pharmacology

Find related publications in this database (Keywords)
lysosomal acid lipase
lipid mediator
Immunology and Microbiology Section
Immune response
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