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SHR Neuro Krebs Kardio Lipid

Hofer, DC; Pessentheiner, AR; Pelzmann, HJ; Schlager, S; Madreiter-Sokolowski, CT; Kolb, D; Eichmann, TO; Rechberger, G; Bilban, M; Graier, WF; Kratky, D; Bogner-Strauss, JG.
Critical role of the peroxisomal protein PEX16 in white adipocyte development and lipid homeostasis.
Biochim Biophys Acta. 2017; 1862(3): 358-368. [OPEN ACCESS]
Web of Science PubMed FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Graier Wolfgang
Kolb-Lenz Dagmar
Kratky Dagmar
Madreiter-Sokolowski Corina
Schlager Stefanie
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Abstract:
The importance of peroxisomes for adipocyte function is poorly understood. Herein, we provide insights into the critical role of peroxin 16 (PEX16)-mediated peroxisome biogenesis in adipocyte development and lipid metabolism. Pex16 is highly expressed in adipose tissues and upregulated during adipogenesis of murine and human cells. We demonstrate that Pex16 is a target gene of the adipogenesis "master-regulator" PPARγ. Stable silencing of Pex16 in 3T3-L1 cells strongly reduced the number of peroxisomes while mitochondrial number was unaffected. Concomitantly, peroxisomal fatty acid (FA) oxidation was reduced, thereby causing accumulation of long- and very long-chain (polyunsaturated) FAs and reduction of odd-chain FAs. Further, Pex16-silencing decreased cellular oxygen consumption and increased FA release. Additionally, silencing of Pex16 impaired adipocyte differentiation, lipogenic and adipogenic marker gene expression, and cellular triglyceride stores. Addition of PPARγ agonist rosiglitazone and peroxisome-related lipid species to Pex16-silenced 3T3-L1 cells rescued adipogenesis. These data provide evidence that PEX16 is required for peroxisome biogenesis and highlights the relevance of peroxisomes for adipogenesis and adipocyte lipid metabolism. Copyright © 2016 The Author(s). Published by Elsevier B.V. All rights reserved.

Find related publications in this database (Keywords)
PEX16
Peroxisome
PPAR gamma
Adipogenesis
Lipid homeostasis
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