Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

Logo MUG-Forschungsportal

Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid

Koyani, CN; Flemmig, J; Malle, E; Arnhold, J.
Myeloperoxidase scavenges peroxynitrite: A novel anti-inflammatory action of the heme enzyme.
Arch Biochem Biophys. 2015; 571(7):1-9 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG


Autor/innen der Med Uni Graz:
Koyani Chintan Navinchandra
Malle Ernst

Dimensions Citations:

Plum Analytics:
Number of Figures: 7
| | | | | | |
Peroxynitrite, a potent pro-inflammatory and cytotoxic species, interacts with a variety of heme containing proteins. We addressed the question whether (i) the interaction of myeloperoxidase (MPO, an enzyme generating hypochlorous acid from hydrogen peroxide and chloride ions) with peroxynitrite affects the clearance of peroxynitrite, and (ii) if peroxynitrite could modulate the chlorinating activity of MPO. Our results show that this interaction promotes the decomposition of the highly reactive pro-inflammatory oxidant, whereby MPO Compound II (but not Compound I) is formed. The efficiency of MPO to remove peroxynitrite was enhanced by L-tyrosine, nitrite and (-)-epicatechin, substances known to reduce Compound II with high reaction rate. Next, peroxynitrite (added as reagent) diminished the chlorinating activity of MPO in the presence of hydrogen peroxide. Alternatively, SIN-1, a peroxynitrite donor, reduced hypochlorous acid formation by MPO, as measured by aminophenyl fluorescein oxidation (time kinetics) and taurine chloramine formation (end point measurement). At inflammatory loci, scavenging of peroxynitrite by MPO may overcome the uncontrolled peroxynitrite decomposition and formation of reactive species, which lead to cell/tissue damage. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.
Find related publications in this database (using NLM MeSH Indexing)
Aniline Compounds - chemistry
Anti-Inflammatory Agents - chemistry
Catechin - chemistry
Fluoresceins - chemistry
Halogenation -
Humans -
Hydrogen Peroxide - chemistry
Hydrogen-Ion Concentration -
Kinetics -
Molsidomine - analogs & derivatives
Molsidomine - chemistry
Nitrites - chemistry
Oxidants - chemistry
Oxidation-Reduction -
Peroxidase - chemistry
Peroxynitrous Acid - chemistry
Tyrosine - chemistry

Find related publications in this database (Keywords)
MPO-H2O2-Cl- system
Nitric oxide
© Med Uni Graz Impressum