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SHR Neuro Krebs Kardio Lipid

Hofmann, NA; Barth, S; Waldeck-Weiermair, M; Klec, C; Strunk, D; Malli, R; Graier, WF.
TRPV1 mediates cellular uptake of anandamide and thus promotes endothelial cell proliferation and network-formation.
Biol Open. 2014; 3(12):1164-1172 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Barth Sonja
Graier Wolfgang
Hofmann Nicole
Klec Christiane
Malli Roland
Strunk Dirk
Waldeck-Weiermair Markus
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Number of Figures: 6
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Abstract:
Anandamide (N-arachidonyl ethanolamide, AEA) is an endogenous cannabinoid that is involved in various pathological conditions, including cardiovascular diseases and tumor-angiogenesis. Herein, we tested the involvement of classical cannabinoid receptors (CBRs) and the Ca(2+)-channel transient receptor potential vanilloid 1 (TRPV1) on cellular AEA uptake and its effect on endothelial cell proliferation and network-formation. Uptake of the fluorescence-labeled anandamide (SKM4-45-1) was monitored in human endothelial colony-forming cells (ECFCs) and a human endothelial-vein cell line (EA.hy926). Involvement of the receptors during AEA translocation was determined by selective pharmacological inhibition (AM251, SR144528, CID16020046, SB366791) and molecular interference by TRPV1-selective siRNA-mediated knock-down and TRPV1 overexpression. We show that exclusively TRPV1 contributes essentially to AEA transport into endothelial cells in a Ca(2+)-independent manner. This TRPV1 function is a prerequisite for AEA-induced endothelial cell proliferation and network-formation. Our findings point to a so far unknown moonlighting function of TRPV1 as Ca(2+)-independent contributor/regulator of AEA uptake. We propose TRPV1 as representing a promising target for development of pharmacological therapies against AEA-triggered endothelial cell functions, including their stimulatory effect on tumor-angiogenesis. © 2014. Published by The Company of Biologists Ltd.

Find related publications in this database (Keywords)
transient receptor potential vanilloid 1
TRPV1
anandamide
AEA
endothelial colony-forming cells
ECFC
anandamide transport
proliferation
network-formation
angiogenesis
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