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Lu, J; Roth, RA; Malle, E; Ganey, PE.
Roles of the hemostatic system and neutrophils in liver injury from co-exposure to amiodarone and lipopolysaccharide.
Toxicol Sci. 2013; 136(1):51-62 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG


Autor/innen der Med Uni Graz:
Malle Ernst

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Number of Figures: 9
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It has been demonstrated that co-treatment of rats with amiodarone (AMD) and bacterial lipopolysaccharide (LPS) produces idiosyncrasy-like liver injury. In this study, the hypothesis that the hemostatic system and neutrophils contribute to AMD/LPS-induced liver injury was explored. Rats were treated with AMD (400 mg/kg, ip) or vehicle and 16 h later with LPS (1.6×10⁶ endotoxin units/kg, iv) or saline (Sal). AMD did not affect the hemostatic system by itself but significantly potentiated LPS-induced coagulation activation and fibrinolysis impairment. Increased hepatic fibrin deposition and subsequent hypoxia were observed only in AMD/LPS-treated animals, starting before the onset of liver injury. Administration of anticoagulant heparin abolished AMD/LPS-induced hepatic fibrin deposition and reduced AMD/LPS-induced liver damage. Polymorphonuclear neutrophils (PMNs) accumulated in liver after treatment with LPS or AMD/LPS, but PMN activation was only observed in AMD/LPS-treated rats. Rabbit anti-rat PMN serum, which reduced accumulation of PMNs in liver, prevented PMN activation and attenuated AMD/LPS-induced liver injury in rats. PMN depletion did not affect hepatic fibrin deposition. Anticoagulation prevented PMN activation without affecting PMN accumulation. In summary, both the hemostatic system alteration and PMN activation contributed to AMD/LPS-induced liver injury in rats, in which fibrin deposition was critical for the activation of PMNs.
Find related publications in this database (using NLM MeSH Indexing)
Amiodarone - toxicity
Animals -
Anoxia - blood
Anticoagulants - pharmacology
Blood Coagulation - drug effects
Drug-Induced Liver Injury - blood
Fibrin - metabolism
Fibrinolysis - drug effects
Hemostasis - drug effects
Heparin - pharmacology
Lipopolysaccharides - toxicity
Liver - drug effects
Male -
Neutrophil Activation - drug effects
Neutrophils - drug effects
Rats -
Rats, Sprague-Dawley -
Time Factors -

Find related publications in this database (Keywords)
idiosyncratic drug-induced liver injury
hemostatic system
hypochlorous acid
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