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Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid

Waltl-Patankar, S; Patankar, J; Fauler, G; Nusshold, C; Ullen, A; Eibinger, G; Wintersperger, A; Kratky, D; Malle, E; Sattler, W.
25-Hydroxycholesterol regulates cholesterol homeostasis in the murine CATH.a neuronal cell line.
Neurosci Lett. 2013; 539(4):16-21 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Eibinger Gerald
Fauler Günter
Kratky Dagmar
Malle Ernst
Nusshold Christoph
Patankar Jay Vasant
Sattler Wolfgang
Üllen Andreas
Waltl Sabine Evelyn
Wintersperger Andrea
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Number of Figures: 5
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Abstract:
Aberrant oxysterol biosynthesis is implicated in the pathogenesis of neurodegenerative diseases. During the present study we have investigated the effects of exogenously added 25-hydroxycholesterol (25-HC) on transcription of cholesterol biosynthetic genes, sterol-regulatory element binding protein (SREBP) processing and cholesterol biosynthesis in the murine CATH.a neuronal cell line. A single i.p. injection of lipopolysaccharide resulted in robust induction of cholesterol 25-hydroxylase mRNA and protein levels in brains of treated mice. In vitro, 25-HC upregulated the transcription of ATP-binding cassette transporter A1 (ABCA1) and (to a lesser extent) apolipoprotein E (apoE) in CATH.a neurons. Cholesterol biosynthetic gene expression (squalene synthase, HMG-CoA synthase, HMG-CoA reductase, and SREBP2) was downregulated by 25-HC. 25-HC also significantly attenuated proteolytic processing of SREBP2. Finally, 25-HC downregulated cholesterol biosynthesis in CATH.a neurons. Our results demonstrate that 25-HC is a potent effector oxysterol of neuronal cholesterol homeostasis.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Cell Line -
Cholesterol - biosynthesis Cholesterol - genetics
Homeostasis -
Hydroxycholesterols - metabolism Hydroxycholesterols - pharmacology
Lipopolysaccharides - pharmacology
Mice -
Neurons - drug effects Neurons - metabolism
RNA, Messenger - metabolism
Steroid Hydroxylases - genetics Steroid Hydroxylases - metabolism
Sterol Regulatory Element Binding Proteins - genetics Sterol Regulatory Element Binding Proteins - metabolism
Transcription, Genetic -

Find related publications in this database (Keywords)
25-Hydroxycholesterol
Cholesterol 25-hydroxylase
Oxysterol
Endotoxin
Neuroinflammation
Neurons
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