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SHR Neuro Krebs Kardio Lipid

Poparic, I; Schreibmayer, W; Schoser, B; Desoye, G; Gorischek, A; Miedl, H; Hochmeister, S; Binder, J; Quasthoff, S; Wagner, K; Windpassinger, C; Malle, E.
Four and a half LIM protein 1C (FHL1C): a binding partner for voltage-gated potassium channel K(v1.5).
PLOS ONE. 2011; 6(10): e26524-e26524. [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Binder Josepha
Desoye Gernot
Gorischek Astrid
Hochmeister Sonja
Malle Ernst
Miedl Heidi
Poparic Ivana
Quasthoff Stefan
Schreibmayer Wolfgang
Wagner Klaus
Windpassinger Christian
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Number of Figures: 7
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Abstract:
Four-and-a-half LIM domain protein 1 isoform A (FHL1A) is predominantly expressed in skeletal and cardiac muscle. Mutations in the FHL1 gene are causative for several types of hereditary myopathies including X-linked myopathy with postural muscle atrophy (XMPMA). We here studied myoblasts from XMPMA patients. We found that functional FHL1A protein is completely absent in patient myoblasts. In parallel, expression of FHL1C is either unaffected or increased. Furthermore, a decreased proliferation rate of XMPMA myoblasts compared to controls was observed but an increased number of XMPMA myoblasts was found in the G(0)/G(1) phase. Furthermore, low expression of K(v1.5), a voltage-gated potassium channel known to alter myoblast proliferation during the G(1) phase and to control repolarization of action potential, was detected. In order to substantiate a possible relation between K(v1.5) and FHL1C, a pull-down assay was performed. A physical and direct interaction of both proteins was observed in vitro. In addition, confocal microscopy revealed substantial colocalization of FHL1C and K(v1.5) within atrial cells, supporting a possible interaction between both proteins in vivo. Two-electrode voltage clamp experiments demonstrated that coexpression of K(v1.5) with FHL1C in Xenopus laevis oocytes markedly reduced K(+) currents when compared to oocytes expressing K(v1.5) only. We here present the first evidence on a biological relevance of FHL1C.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Blotting, Western -
Case-Control Studies -
Cell Cycle -
Cell Line -
Cell Proliferation -
Genetic Diseases, X-Linked - metabolism Genetic Diseases, X-Linked - pathology
Humans -
Intracellular Signaling Peptides and Proteins - genetics Intracellular Signaling Peptides and Proteins - metabolism
Ion Channel Gating -
Kinetics -
Kv1.5 Potassium Channel - genetics Kv1.5 Potassium Channel - metabolism
LIM Domain Proteins - genetics LIM Domain Proteins - metabolism
Male -
Mice -
Muscle Proteins - genetics Muscle Proteins - metabolism
Muscular Atrophy - metabolism Muscular Atrophy - pathology
Mutant Proteins - metabolism
Myoblasts - metabolism Myoblasts - pathology
Protein Binding -
Protein Isoforms - genetics Protein Isoforms - metabolism
Protein Transport -
Reverse Transcriptase Polymerase Chain Reaction -
Subcellular Fractions - metabolism
Xenopus -
Xenopus laevis -

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