Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

Logo MUG-Forschungsportal

Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid

Fauland, A; Köfeler, H; Trötzmüller, M; Knopf, A; Hartler, J; Eberl, A; Chitraju, C; Lankmayr, E; Spener, F.
A comprehensive method for lipid profiling by liquid chromatography-ion cyclotron resonance mass spectrometry.
J Lipid Res. 2011; 52(12):2314-2322 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Hartler Jürgen
Knopf Astrid
Köfeler Harald
Spener Friedrich
Trötzmüller Martin
Altmetrics:

Dimensions Citations:

Plum Analytics:
Number of Figures: 8
| | | | | | | |
Abstract:
This work aims to combine chromatographic retention, high mass resolution and accuracy, MS/MS spectra, and a package for automated identification and quantitation of lipid species in one platform for lipidomic analysis. The instrumental setup elaborated comprises reversed-phase HPLC coupled to a Fourier transform ion cyclotron resonance mass spectrometer (LTQ-FT), and Lipid Data Analyzer (LDA) software. Data analysis for lipid species quantification in this platform is based on retention time, mass resolution of 200,000, and mass accuracy below 2 ppm. In addition, automatically generated MS/MS spectra provide structural information at molecular level. This LC/MS technology allows analyzing complex biological samples in a quantitative manner as shown here paradigmatically for murine lipid droplets having a huge surplus of triacylglycerol species. Chromatographic preseparation of the bulk lipid class alleviates the problem of ion suppression of lipid species from other classes. Extension of 1D to 2D chromatography is possible, yet time consuming. The platform affords unambiguous detection of lipid species as low as 0.1‰ within major lipid classes. Taken together, a novel lipidomic LC/MS platform based on chromatographic retention, high mass resolution and accuracy, MS/MS analysis, and quantitation software enables analysis of complex samples as demonstrated for lipid droplets.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Chromatography, High Pressure Liquid - methods
Chromatography, Reverse-Phase -
Cyclotrons -
Lipids - analysis
Mice -
Mice, Inbred C57BL -
Reproducibility of Results -
Tandem Mass Spectrometry - instrumentation
Time Factors -

Find related publications in this database (Keywords)
high-resolution mass spectrometry
lipidomics
HPLC/ESI-MS
HILIC
lipid droplets
triacylglycerols
phospholipids
© Meduni Graz Impressum