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SHR Neuro Krebs Kardio Lipid

Wagner, V; Stadelmeyer, E; Riederer, M; Regitnig, P; Gorischek, A; Devaney, T; Schmidt, K; Tritthart, HA; Hirschberg, K; Bauernhofer, T; Schreibmayer, W.
Cloning and characterisation of GIRK1 variants resulting from alternative RNA editing of the KCNJ3 gene transcript in a human breast cancer cell line.
J Cell Biochem. 2010; 110(3): 598-608.
Web of Science PubMed FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Bauernhofer Thomas
Devaney Trevor
Gorischek Astrid
Regitnig Peter
Riederer Monika
Schreibmayer Wolfgang
Stadelmeyer Elke
Wagner Valerie
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Abstract:
The aim of this study was to investigate the impact of increased mRNA levels encoding GIRK1 in breast tumours on GIRK protein expression. mRNA levels encoding hGIRK1 and hGIRK4 in the MCF7, MCF10A and MDA-MB-453 breast cancer cell lines were assessed and the corresponding proteins detected using Western blots. cDNAs encoding for four hGIRK1 splice variants (hGIRK1a, 1c, 1d and 1e) were cloned from the MCF7 cell line. Subcellular localisation of fluorescence labelled hGIRK1a-e and hGIRK4 and of endogenous GIRK1 and GIRK4 subunits was monitored in the MCF7 cell line. All hGIRK1 splice variants and hGIRK4 were predominantly located within the endoplasmic reticulum. Heterologous expression in Xenopus laevis oocytes and two electrode voltage clamp experiments together with confocal microscopy were performed. Only the hGIRK1a subunit was able to form functional GIRK channels in connection with hGIRK4. The other splice variants are expressed, but exert a dominant negative effect on heterooligomeric channel function. Hence, alternative splicing of the KCNJ3 gene transcript in the MCF7 cell line leads to a family of mRNA's, encoding truncated versions of the hGIRK1 protein. The very high abundance of mRNA's encoding GIRK1 together with the presence of GIRK1 protein suggests a pathophysiological role in breast cancer. (c) 2010 Wiley-Liss, Inc.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Base Sequence -
Blotting, Western -
Breast Neoplasms - genetics
Cell Line, Tumor -
Clone Cells -
Female -
G Protein-Coupled Inwardly-Rectifying Potassium Channels - genetics
Humans -
Immunohistochemistry -
Microscopy, Confocal -
Molecular Sequence Data -
Patch-Clamp Techniques -
Protein Isoforms - genetics
RNA Editing - genetics
RNA, Messenger - analysis
Reverse Transcriptase Polymerase Chain Reaction -
Xenopus laevis -

Find related publications in this database (Keywords)
GIRK1
GIRK4
BREAST CANCER
MCF7
MCF10A
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