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SHR Neuro Krebs Kardio Lipid

Krause, I; Pohler, U; Grosse, S; Shebl, O; Petek, E; Chandra, A; Ebner, T.
Characterization of the injection funnel during intracytoplasmic sperm injection reflects cytoplasmic maturity of the oocyte.
Fertil Steril. 2016; 106(5): 1101-1106.
Web of Science PubMed FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Ebner Thomas
Petek Erwin
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Abstract:
To quantify cytoplasmic maturity on the basis of intracytoplasmic sperm injection (ICSI) injection funnel manifestation and to evaluate influence factors of the latter. Prospective study. Private fertility center. A total of 31 patients with good ovarian response. Mature and immature oocytes were injected intracytoplasmatically. Formation and persistence of an injection funnel was documented and measured. ICSI funnel size, persistence of injection funnel, rates of degeneration and fertilization, embryo quality. Funnel volume in germinal vesicle stage oocytes (prophase I [PI]) was significantly smaller than that of metaphase I (MI) and MII oocytes. Immature eggs (PI, MI) almost never showed a persistent funnel 2-4 minutes after ICSI, whereas in MII eggs the funnel was still observable in 35% (117/334) of the cases. Uni- and multivariate analysis revealed that pipette type and stimulation protocol significantly influenced appearance of injection funnel. Funnel volume in oocytes that fertilized regularly was significantly higher compared with three-polar body and degenerated oocytes. Oocyte maturation within the follicle is closely associated with a remarkable change in cytoplasm viscosity from an aqueous to a more viscous subtype. Precise evaluation of the injection funnel may help to explain deviations from expected ICSI outcome and could also assist in optimizing controlled ovarian hyperstimulation. Copyright © 2016 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

Find related publications in this database (Keywords)
Injection funnel
ICSI
ICSI pipette
cytoplasmic maturity
controlled ovarian hyperstimulation
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