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SHR Neuro Krebs Kardio Lipid

Rossmann, C; Windpassinger, C; Brunner, D; Kovacevic, A; Schweighofer, N; Malli, R; Schuligoi, R; Prokesch, A; Kluve-Beckerman, B; Graier, WF; Kratky, D; Sattler, W; Malle, E.
Characterization of rat serum amyloid A4 (SAA4): A novel member of the SAA superfamily.
Biochem Biophys Res Commun. 2014; 450(4):1643-1649 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Graier Wolfgang
Kovacevic Alenka
Kratky Dagmar
Malle Ernst
Malli Roland
Prokesch Andreas
Rossmann Christine Renate
Sattler Wolfgang
Schuligoi Rufina
Schweighofer Natascha
Windpassinger Christian
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Number of Figures: 7
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Abstract:
The serum amyloid A (SAA) family of proteins is encoded by multiple genes, which display allelic variation and a high degree of homology in mammals. The SAA1/2 genes code for non-glycosylated acute-phase SAA1/2 proteins, that may increase up to 1000-fold during inflammation. The SAA4 gene, well characterized in humans (hSAA4) and mice (mSaa4) codes for a SAA4 protein that is glycosylated only in humans. We here report on a previously uncharacterized SAA4 gene (rSAA4) and its product in Rattus norvegicus, the only mammalian species known not to express acute-phase SAA. The exon/intron organization of rSAA4 is similar to that reported for hSAA4 and mSaa4. By performing 5'- and 3'RACE, we identified a 1830-bases containing rSAA4 mRNA (including a GA-dinucleotide tandem repeat). Highest rSAA4 mRNA expression was detected in rat liver. In McA-RH7777 rat hepatoma cells, rSAA4 transcription was significantly upregulated in response to LPS and IL-6 while IL-1α/β and TNFα were without effect. Luciferase assays with promoter-truncation constructs identified three proximal C/EBP-elements that mediate expression of rSAA4 in McA-RH7777 cells. In line with sequence prediction a 14-kDa non-glycosylated SAA4 protein is abundantly expressed in rat liver. Fluorescence microscopy revealed predominant localization of rSAA4-GFP-tagged fusion protein in the ER. Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.

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