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Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid

El-Heliebi, A; Kroneis, T; Zöhrer, E; Haybaeck, J; Fischereder, K; Kampel-Kettner, K; Zigeuner, R; Pock, H; Riedl, R; Stauber, R; Geigl, JB; Huppertz, B; Sedlmayr, P; Lackner, C.
Are morphological criteria sufficient for the identification of circulating tumor cells in renal cancer?
J Transl Med. 2013; 11(10):214-214 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
El-Heliebi Amin
Fischereder Katja
Geigl Jochen Bernd
Haybäck Johannes
Huppertz Berthold
Kroneis Thomas
Lackner Karoline
Pock Hannelore
Riedl Regina
Sedlmayr Peter
Stauber Rudolf
Strini Karin
Zigeuner Richard
Zöhrer Evelyn
Gendermonitor:
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Number of Figures: 10
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Abstract:
Single circulating tumor cells (CTCs) or circulating tumor microemboli (CTMs) are potential biomarkers of renal cell cancer (RCC), however studies of CTCs/CTMs in RCC are limited. In this pilot study we aimed to evaluate a novel blood filtration technique suited for cytomorphological classification, immunocytochemical and molecular characterization of filtered, so called circulating non-hematologic cells (CNHCs) - putative CTCs/CTMs - in patients with RCC. Blood of 40 patients with renal tumors was subjected to ScreenCell filtration. CNHCs were classified according to cytomorphological criteria. Immunocytochemical analysis was performed with antibodies against CD45, CD31 and carbonic anhydrase IX (CAIX, a RCC marker). DNA of selected CNHCs and respective primary tumors was analysed by array-CGH. CNHC-clusters with malignant or uncertain malignant cytomorphological features - putative CTMs - were negative for CD45, positive for CD31, while only 6% were CAIX positive. Array-CGH revealed that 83% of malignant and uncertain malignant cells did represent with a balanced genome whereas 17% presented genomic DNA imbalances which did not match the aberrations of the primary tumors. Putative single CTCs were negative for CD45, 33% were positive for CD31 and 56% were positive for CAIX. The majority of CNHC-clusters, putative CTMs, retrieved by ScreenCell filtration may be of endothelial origin. Morphological criteria seem to be insufficient to distinguish malignant from non-malignant cells in renal cancer.
Find related publications in this database (using NLM MeSH Indexing)
Adult -
Aged -
Aged, 80 and over -
Antigens, CD31 - metabolism
Biomarkers, Tumor - metabolism
Carcinoma, Renal Cell - metabolism
Case-Control Studies -
Cell Count -
Cell Shape -
Cohort Studies -
Comparative Genomic Hybridization -
DNA, Neoplasm - metabolism
Female -
Humans -
Immunohistochemistry -
Kidney Neoplasms - pathology
Laser Capture Microdissection -
Male -
Middle Aged -
Neoplastic Cells, Circulating - pathology
Time Factors -

Find related publications in this database (Keywords)
Circulating tumor cells
Circulating tumor microemboli
Renal cancer
ScreenCell (R)
Array comparative genomic hybridization (array-CGH)
Circulating endothelial cells
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