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Balic, M; Schwarzenbacher, D; Stanzer, S; Heitzer, E; Auer, M; Geigl, JB; Cote, RJ; Datar, RH; Dandachi, N.
Genetic and epigenetic analysis of putative breast cancer stem cell models.
BMC Cancer. 2013; 13(7):358-358 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Auer Martina
Balic Marija
Dandachi Nadia
Geigl Jochen Bernd
Heitzer Ellen
Schwarzenbacher Daniela
Stanzer Stefanie
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Number of Figures: 10
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Abstract:
Cancer stem cell model hypothesizes existence of a small proportion of tumor cells capable of sustaining tumor formation, self-renewal and differentiation. In breast cancer, these cells were found to be associated with CD44⁺CD24-low and ALDH⁺ phenotype. Our study was performed to evaluate the suitability of current approaches for breast cancer stem cell analyses to evaluate heterogeneity of breast cancer cells through their extensive genetic and epigenetic characterization. Breast cancer cell lines MCF7 and SUM159 were cultured in adherent conditions and as mammospheres. Flow cytometry sorting for CD44, CD24 and ALDH was performed. Sorted and unsorted populations, mammospheres and adherent cell cultures were subjected to DNA profiling by array CGH and methylation profiling by Epitect Methyl qPCR array. Methylation status of selected genes was further evaluated by pyrosequencing. Functional impact of methylation was evaluated by mRNA analysis for selected genes. Array CGH did not reveal any genomic differences. In contrast, putative breast cancer stem cells showed altered methylation levels of several genes compared to parental tumor cells. Our results underpin the hypothesis that epigenetic mechanisms seem to play a major role in the regulation of CSCs. However, it is also clear that more efficient methods for CSC enrichment are needed. This work underscores requirement of additional approaches to reveal heterogeneity within breast cancer.
Find related publications in this database (using NLM MeSH Indexing)
Antigens, CD24 - analysis
Breast Neoplasms - genetics
Cell Line, Tumor -
Comparative Genomic Hybridization -
DNA Methylation - genetics
Epigenesis, Genetic -
Female -
Flow Cytometry -
Gene Expression Regulation, Neoplastic -
Humans -
Neoplastic Stem Cells -
Real-Time Polymerase Chain Reaction -
Transcriptome -

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