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SHR Neuro Krebs Kardio Lipid

Daniele, T; Hackmann, Y; Ritter, AT; Wenham, M; Booth, S; Bossi, G; Schintler, M; Auer-Grumbach, M; Griffiths, GM.
A role for rab7 in the movement of secretory granules in cytotoxic T lymphocytes.
Traffic. 2011; 12(7): 902-911. [OPEN ACCESS]
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Autor/innen der Med Uni Graz:
Auer-Grumbach Michaela
Schintler Michael
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Number of Figures: 6
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Abstract:
Cytotoxic T lymphocytes (CTL) are potent killers of virally infected and tumorigenic cells. Upon recognition of target cells, CTL undergo polarized secretion of secretory lysosomes at the immunological synapse (IS) that forms between CTL and target. However, the molecular machinery involved in the polarization of secretory lysosomes is still largely uncharacterized. In this paper, we investigated the role of Rab7 in the polarization of secretory lysosomes. We show that silencing of Rab7 by RNA interference reduces the ability of CTL to kill targets. GTP-bound Rab7 and Rab interacting lysosomal protein, RILP, interact and both localize to secretory lysosomes in CTL. Over-expression of RILP recruits dynein to the membranes of secretory lysosomes and triggers their movement toward the centrosome. Together, these results suggest that Rab7 may play a role in secretory lysosome movement toward the centrosome by interacting with RILP to recruit the minus-end motor, dynein.
Find related publications in this database (using NLM MeSH Indexing)
Adaptor Proteins, Signal Transducing - genetics
Animals -
Carrier Proteins - metabolism
Cytoskeletal Proteins - metabolism
Dyneins - metabolism
Guanosine Triphosphate - metabolism
HeLa Cells -
Humans -
Lysosomes - metabolism
Mice -
Mice, Inbred C57BL -
Mutation -
RNA Interference -
Recombinant Fusion Proteins - genetics
Secretory Vesicles - metabolism
T-Lymphocytes, Cytotoxic - metabolism
rab GTP-Binding Proteins - genetics

Find related publications in this database (Keywords)
Bicaudal
CTL
CMT2B
dynein
lymphocytes
lysosomes
ORP1L
polarization
Rab7
RILP
secretion
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