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Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid

Chen, S; El-Heliebi, A; Tauber, G; Langsenlehner, T; Pötscher, M; Kashofer, K; Czyż, ZT; Polzer, B; Riethdorf, S; Kuske, A; Leitinger, G; Pantel, K; Kroneis, T; Sedlmayr, P.
Catch and Release: rare cell analysis from a functionalised medical wire.
Sci Rep. 2017; 7(2):43424-43424 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Chen Shukun
El-Heliebi Amin
Kashofer Karl
Kroneis Thomas
Langsenlehner Tanja
Leitinger Gerd
Sedlmayr Peter
Tauber Gerlinde
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Number of Figures: 3
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Abstract:
Enumeration and especially molecular characterization of circulating tumour cells (CTCs) holds great promise for cancer management. We tested a modified type of an in vivo enrichment device (Catch&Release) for its ability to bind and detach cancer cells for the purpose of single-cell molecular downstream analysis in vitro. The evaluation showed that single-cell analysis using array comparative genome hybridization (array-CGH) and next generation sequencing (NGS) is feasible. We found array-CGH to be less noisy when whole genome amplification (WGA) was performed with Ampli1 as compared to GenomePlex (DLRS values 0.65 vs. 1.39). Moreover, Ampli1-processed cells allowed detection of smaller aberrations (median 14.0 vs. 49.9 Mb). Single-cell NGS data obtained from Ampli1-processed samples showed the expected non-synonymous mutations (deletion/SNP) according to bulk DNA. We conclude that clinical application of this refined in vivo enrichment device allows CTC enumeration and characterization, thus, representing a promising tool for personalized medicine.
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Cell Separation - instrumentation
Cell Separation - methods
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Epithelial Cell Adhesion Molecule - genetics
Epithelial Cell Adhesion Molecule - metabolism
Equipment Design -
Gene Expression -
High-Throughput Nucleotide Sequencing -
Humans -
Neoplasms - diagnosis
Neoplasms - genetics
Neoplasms - metabolism
Neoplasms - pathology
Neoplastic Cells, Circulating - metabolism
Neoplastic Cells, Circulating - pathology
Precision Medicine -
Single-Cell Analysis - instrumentation
Single-Cell Analysis - methods

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