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Grisold, AJ; Zarfel, G; Strenger, V; Feierl, G; Leitner, E; Masoud, L; Hoenigl, M; Raggam, RB; Dosch, V; Marth, E.
Use of automated repetitive-sequence-based PCR for rapid laboratory confirmation of nosocomial outbreaks.
J Infect. 2010; 60(1):44-51
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Autor/innen der Med Uni Graz:
Feierl Gebhard
Grisold Andrea
Hönigl Martin
Leitner-Meyer Eva
Marth Egon
Masoud-Landgraf Lilian
Raggam Reinhard Bernd
Strenger Volker
Zarfel Gernot
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Abstract:
Rapid and reliable diagnosis of genetic relatedness of clinical isolates in microbiologic laboratory is essential in case of nosocomial outbreak investigation. Most molecular techniques used to type microorganisms are technically demanding and time consuming. Currently repetitive-sequence-based PCR (rep-PCR) technique has been adapted to an automated format on the DiversiLab system (bioMérieux, Marcy l'Etoile, France). Aim of this study was to compare the performance of the DiversiLab system to that of pulsed-field gel electrophoresis (PFGE) in nosocomial outbreaks. 122 clinical isolates (28 Methicillin-resistant Staphylococcus aureus (MRSA), 26 Acinetobacter baumannii, 45 extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae and 13 ESBL-producing Klebsiella oxytoca) were investigated. 70 isolates originated from six well-documented outbreaks, 52 were non-outbreak isolates. Concordant results for identification of outbreak and non-outbreak MRSA, A. baumannii and ESBL-producing K. pneumoniae strains were achieved with both methods. In the outbreak of ESBL-producing K. oxytoca automated rep-PCR was slightly more discriminatory than PFGE. Rep-PCR identified investigated ESBL-producing K. oxytoca outbreak-strains as indistinguishable or closely related, showing similarity of >90%, while PFGE identified these strains as indistinguishable. Automated rep-PCR assays on the DiversiLab system were used for MRSA, A. baumannii and for the first time ESBL-producing Klebsiella spp. and proved as a rapid and reliable method for molecular analysis of nosocomial outbreaks. 2009 The British Infection Society. Published by Elsevier Ltd. All rights reserved.
Find related publications in this database (using NLM MeSH Indexing)
Acinetobacter Infections - diagnosis Acinetobacter Infections - epidemiology Acinetobacter Infections - genetics
Acinetobacter baumannii - genetics Acinetobacter baumannii - isolation & purification
Bacteria - classification Bacteria - genetics Bacteria - isolation & purification
Bacterial Infections - diagnosis
Cross Infection - diagnosis Cross Infection - epidemiology
DNA, Bacterial - chemistry
Disease Outbreaks -
Humans -
Klebsiella Infections - diagnosis Klebsiella Infections - epidemiology Klebsiella Infections - genetics
Klebsiella oxytoca - genetics Klebsiella oxytoca - isolation & purification
Klebsiella pneumoniae - genetics Klebsiella pneumoniae - isolation & purification
Methicillin-Resistant Staphylococcus aureus - genetics Methicillin-Resistant Staphylococcus aureus - isolation & purification
Polymerase Chain Reaction - methods
Repetitive Sequences, Nucleic Acid -
Staphylococcal Infections - diagnosis Staphylococcal Infections - epidemiology

Find related publications in this database (Keywords)
Methicillin-resistant Staphylococcus aureus (MRSA)
Acinetobacter spp.
Extended-spectrum beta-lactamase-producing Klebsiella
pneumoniae
Extended-spectrum beta-lactamase-producing Klebsiella
oxytoca
Automated repetitive-sequence-based PCR
DiversiLab Microbial Typing System (TM)
Nosocomial outbreak
Surveillance
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