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SHR Neuro Krebs Kardio Lipid

Regauer, S; Beham-Schmid, C.
Detailed analysis of the T-cell lymphocytic infiltrate in penile lichen sclerosus: an immunohistochemical and molecular investigation.
Histopathology. 2006; 48(6):730-735
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Autor/innen der Med Uni Graz:
Beham-Schmid Christine
Regauer Sigrid

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AIM: To determine the extent of clonal outgrowth in the lymphocytic tissue infiltrate of lichen sclerosus (LS). The presence of T cells with a monoclonally rearranged T-cell receptor gamma-gene (TCRgamma) has been described in up to 50% of biopsies of vulvar and penile LS. MATERIAL AND RESULTS: We analysed 33 foreskin specimens with LS for the presence of clonal T cells by conventional polymerase chain reaction (PCR) analysis and with TCRgamma-PCR-based fluorescent fragment analysis. Eighteen of 33 patients revealed a band indicating a monoclonally rearranged TCRgamma on conventional PCR analysis. Subsequent TCRgamma-PCR-based fluorescent fragment analysis identified 8/18 patients with monoclonal T-cell DNA ranging from 1.4% to 23.1% of total T-cell DNA analysed and a size range from 56 to 72 base pairs. Four of 18 patients had an oligoclonal and 6/18 patients revealed a polyclonal banding pattern. The lymphocytic infiltrate contained low numbers of gammadelta T cells and cytotoxic T cells in comparable numbers to the low percentage of clonal TCRgamma DNA. CONCLUSIONS: The low percentage of clonal TCRgamma DNA argues against a systemic neoplastic disease, but rather for a local immune disorder. The target antigen of the clonal outgrowth is unknown, but an exaggerated antigen-dependent proliferation of T cells due to chronic local antigen exposure, probably an infectious antigen, is the most likely explanation.
Find related publications in this database (using NLM MeSH Indexing)
Adolescent -
Adult -
Aged -
Antigens, CD3 - analysis
Antigens, CD4 - analysis
Antigens, CD7 - analysis
Antigens, CD8 - analysis
Child - analysis
Child, Preschool - analysis
Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor - genetics
Humans - genetics
Immunohistochemistry - genetics
Lichen Sclerosus et Atrophicus - immunology
Male - immunology
Middle Aged - immunology
Penile Diseases - immunology
Polymerase Chain Reaction - immunology
T-Lymphocytes - chemistry

Find related publications in this database (Keywords)
immune dysregulation
lymphoid infiltrate
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