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SHR Neuro Krebs Kardio Lipid Stoffw Microb

Matzer, I; Voglhuber, J; Kiessling, M; Djalinac, N; Trummer-Herbst, V; Mabotuwana, N; Rech, L; Holzer, M; Sossalla, S; Rainer, PP; Zirlik, A; Ljubojevic-Holzer, S.
β-Adrenergic Receptor Stimulation Maintains NCX-CaMKII Axis and Prevents Overactivation of IL6R-Signaling in Cardiomyocytes upon Increased Workload.
Biomedicines. 2022; 10(7): Doi: 10.3390/biomedicines10071648 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG


Führende Autor*innen der Med Uni Graz
Holzer Senka
Matzer Ingrid
Voglhuber Julia
Co-Autor*innen der Med Uni Graz
Dalinac Natasa
Holzer Michael
Mabotuwana Nishani Sandunika
Rainer Peter
Rech Cara Lavinia Shirin
Trummer-Herbst Viktoria
Zirlik Andreas

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Excessive β-adrenergic stimulation and tachycardia are potent triggers of cardiac remodeling; however, their exact cellular effects remain elusive. Here, we sought to determine the potency of β-adrenergic stimulation and tachycardia to modulate gene expression profiles of cardiomyocytes. Using neonatal rat ventricular cardiomyocytes, we showed that tachycardia caused a significant upregulation of sodium-calcium exchanger (NCX) and the activation of calcium/calmodulin-dependent kinase II (CaMKII) in the nuclear region. Acute isoprenaline treatment ameliorated NCX-upregulation and potentiated CaMKII activity, specifically on the sarcoplasmic reticulum and the nuclear envelope, while preincubation with the β-blocker propranolol abolished both isoprenaline-mediated effects. On a transcriptional level, screening for hypertrophy-related genes revealed tachycardia-induced upregulation of interleukin-6 receptor (IL6R). While isoprenaline prevented this effect, pharmacological intervention with propranolol or NCX inhibitor ORM-10962 demonstrated that simultaneous CaMKII activation on the subcellular Ca2+ stores and prevention of NCX upregulation are needed for keeping IL6R activation low. Finally, using hypertensive Dahl salt-sensitive rats, we showed that blunted β-adrenergic signaling is associated with NCX upregulation and enhanced IL6R signaling. We therefore propose a previously unrecognized protective role of β-adrenergic signaling, which is compromised in cardiac pathologies, in preventing IL6R overactivation under increased workload. A better understanding of these processes may contribute to refinement of therapeutic options for patients receiving β-blockers.

Find related publications in this database (Keywords)
beta-adrenergic signaling
hypertensive cardiomyopathy
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