Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Schratter, G; Scheruebel, S; Langthaler, S; Ester, K; Pelzmann, B; Ghaffari-Tabrizi-Wizsy, N; Rezania, S; Gorischek, A; Platzer, D; Zorn-Pauly, K; Ahammer, H; Prokesch, A; Stanzer, S; Devaney, TTJ; Schmidt, K; Jahn, SW; Prassl, R; Bauernhofer, T; Schreibmayer, W; .
GIRK1 triggers multiple cancer-related pathways in the benign mammary epithelial cell line MCF10A.
SCI REP. 2019; 9: 19277
Doi: 10.1038/s41598-019-55683-w
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- Führende Autor*innen der Med Uni Graz
- Schratter Gebhard
- Schreibmayer Wolfgang
- Co-Autor*innen der Med Uni Graz
- Ahammer Helmut
- Bauernhofer Thomas
- DeVaney Trevor
- Ghaffari Tabrizi-Wizsy Nassim
- Gorischek Astrid
- Jahn Stephan
- Pelzmann Brigitte
- Platzer Dieter
- Prassl Ruth
- Prokesch Andreas
- Rezania Simin
- Scherübel-Posch Susanne
- Stanzer Stefanie
- Zorn-Pauly Klaus
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- Abstract:
- Excessive expression of subunit 1 of GIRK1 in ER+ breast tumors is associated with reduced survival times and increased lymph node metastasis in patients. To investigate possible tumor-initiating properties, benign MCF10A and malign MCF7 mammary epithelial cells were engineered to overexpress GIRK1 neoplasia associated vital parameters and resting potentials were measured and compared to controls. The presence of GIRK1 resulted in resting potentials negative to the controls. Upon GIRK1 overexpression, several cellular pathways were regulated towards pro-tumorigenic action as revealed by comparison of transcriptomes of MCF10AGIRK1 with the control (MCF10AeGFP). According to transcriptome analysis, cellular migration was promoted while wound healing and extracellular matrix interactions were impaired. Vital parameters in MCF7 cells were affected akin the benign MCF10A lines, but to a lesser extent. Thus, GIRK1 regulated cellular pathways in mammary epithelial cells are likely to contribute to the development and progression of breast cancer.