Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Waldeck-Weiermair, M; Gottschalk, B; Madreiter-Sokolowski, CT; Ramadani-Muja, J; Ziomek, G; Klec, C; Burgstaller, S; Bischof, H; Depaoli, MR; Eroglu, E; Malli, R; Graier, WF.
Development and Application of Sub-Mitochondrial Targeted Ca2 + Biosensors.
Front Cell Neurosci. 2019; 13: 449-449.
Doi: 10.3389/fncel.2019.00449
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- Führende Autor*innen der Med Uni Graz
- Waldeck-Weiermair Markus
- Co-Autor*innen der Med Uni Graz
- Bischof Helmut
- Burgstaller Sandra
- Depaoli Maria Rosa
- EROGLU Emrah
- Gottschalk Benjamin
- Graier Wolfgang
- Klec Christiane
- Madreiter-Sokolowski Corina
- Malli Roland
- Ramadani-Muja Jeta
- Ziomek Gabriela
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- Abstract:
- Mitochondrial Ca2+ uptake into the mitochondrial matrix is a well-established mechanism. However, the sub-organellar Ca2+ kinetics remain elusive. In the present work we identified novel site-specific targeting sequences for the intermembrane space (IMS) and the cristae lumen (CL). We used these novel targeting peptides to develop green- and red- Ca2+ biosensors targeted to the IMS and to the CL. Based on their distinctive spectral properties, and comparable sensitivities these novel constructs were suitable to visualize Ca2+-levels in various (sub) compartments in a multi-chromatic manner. Functional studies that applied these new biosensors revealed that knockdown of MCU and EMRE yielded elevated Ca2+ levels inside the CL but not the IMS in response to IP3-generating agonists. Knockdown of VDAC1, however, strongly impeded the transfer of Ca2+ through the OMM while the cytosolic Ca2+ signal remained unchanged. The novel sub-mitochondrially targeted Ca2+ biosensors proved to be suitable for Ca2+ imaging with high spatial and temporal resolution in a multi-chromatic manner allowing simultaneous measurements. These informative biosensors will facilitate efforts to dissect the complex sub-mitochondrial Ca2+ signaling under (patho)physiological conditions. Copyright © 2019 Waldeck-Weiermair, Gottschalk, Madreiter-Sokolowski, Ramadani-Muja, Ziomek, Klec, Burgstaller, Bischof, Depaoli, Eroglu, Malli and Graier.
- Find related publications in this database (Keywords)
- Ca2+ measurements
- biosensors
- mitochondrial Ca2+
- mitochondrial cristae
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- live cell imaging
- mitochondria