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SHR Neuro Krebs Kardio Lipid Stoffw Microb

Mayr, C; Beyreis, M; Dobias, H; Gaisberger, M; Fuchs, J; Pichler, M; Ritter, M; Jakab, M; Helm, K; Neureiter, D; Kiesslich, T.
Continuous, label-free, 96-well-based determination of cell migration using confluence measurement.
Cell Adh Migr. 2019; 13(1):76-82 Doi: 10.1080/19336918.2018.1526612 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG


Co-Autor*innen der Med Uni Graz
Pichler Martin

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Cellular migration is essential in diverse physiological and pathophysiological processes. Here, we present a protocol for quantitative analysis of migration using confluence detection allowing continuous, non-endpoint measurement with minimal hands-on time under cell incubator conditions. Applicability was tested using substances which enhance (EGF) or inhibit (cytochalasin D, ouabain) migration. Using a gap-closure assay we demonstrate that automated confluence detection monitors cellular migration in the 96-well microplate format. Quantification by % confluence, % cell free-area or % confluence in cell-free area against time, allows detailed analysis of cellular migration. The study describes a practicable approach for continuous, non-endpoint measurement of migration in 96-well microplates and for detailed data analysis, which allows for medium/high-throughput analysis of cellular migration in vitro.

Find related publications in this database (Keywords)
gap-closure assay
confluence measurement
continuous measurement
cytochalasin D
epidermal growth factor
A549 human lung carcinoma cells
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