Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Mayr, C; Beyreis, M; Dobias, H; Gaisberger, M; Fuchs, J; Pichler, M; Ritter, M; Jakab, M; Helm, K; Neureiter, D; Kiesslich, T.
Continuous, label-free, 96-well-based determination of cell migration using confluence measurement.
Cell Adh Migr. 2019; 13(1):76-82
Doi: 10.1080/19336918.2018.1526612
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- Co-Autor*innen der Med Uni Graz
- Pichler Martin
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- Abstract:
- Cellular migration is essential in diverse physiological and pathophysiological processes. Here, we present a protocol for quantitative analysis of migration using confluence detection allowing continuous, non-endpoint measurement with minimal hands-on time under cell incubator conditions. Applicability was tested using substances which enhance (EGF) or inhibit (cytochalasin D, ouabain) migration. Using a gap-closure assay we demonstrate that automated confluence detection monitors cellular migration in the 96-well microplate format. Quantification by % confluence, % cell free-area or % confluence in cell-free area against time, allows detailed analysis of cellular migration. The study describes a practicable approach for continuous, non-endpoint measurement of migration in 96-well microplates and for detailed data analysis, which allows for medium/high-throughput analysis of cellular migration in vitro.
- Find related publications in this database (Keywords)
- Migration
- gap-closure assay
- confluence measurement
- non-endpoint
- high-throughput
- continuous measurement
- cytochalasin D
- ouabain
- epidermal growth factor
- A549 human lung carcinoma cells