Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Alesutan, I; Voelkl, J; Feger, M; Kratschmar, DV; Castor, T; Mia, S; Sacherer, M; Viereck, R; Borst, O; Leibrock, C; Gawaz, M; Kuro-O, M; Pilz, S; Tomaschitz, A; Odermatt, A; Pieske, B; Wagner, CA; Lang, F.
Involvement Of Vascular Aldosterone Synthase In Phosphate-Induced Osteogenic Transformation Of Vascular Smooth Muscle Cells.
Sci Rep. 2017; 7(1):2059-2059
Doi: 10.1038/s41598-017-01882-2
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- Co-Autor*innen der Med Uni Graz
- Pieske Burkert Mathias
- Pilz Stefan
- Sacherer Michael
- Tomaschitz Andreas
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- Abstract:
- Vascular calcification resulting from hyperphosphatemia is a major determinant of mortality in chronic kidney disease (CKD). Vascular calcification is driven by aldosterone-sensitive osteogenic transformation of vascular smooth muscle cells (VSMCs). We show that even in absence of exogenous aldosterone, silencing and pharmacological inhibition (spironolactone, eplerenone) of the mineralocorticoid receptor (MR) ameliorated phosphate-induced osteo-/chondrogenic transformation of primary human aortic smooth muscle cells (HAoSMCs). High phosphate concentrations up-regulated aldosterone synthase (CYP11B2) expression in HAoSMCs. Silencing and deficiency of CYP11B2 in VSMCs ameliorated phosphate-induced osteogenic reprogramming and calcification. Phosphate treatment was followed by nuclear export of APEX1, a CYP11B2 transcriptional repressor. APEX1 silencing up-regulated CYP11B2 expression and stimulated osteo-/chondrogenic transformation. APEX1 overexpression blunted the phosphate-induced osteo-/chondrogenic transformation and calcification of HAoSMCs. Cyp11b2 expression was higher in aortic tissue of hyperphosphatemic klotho-hypomorphic (kl/kl) mice than in wild-type mice. In adrenalectomized kl/kl mice, spironolactone treatment still significantly ameliorated aortic osteoinductive reprogramming. Our findings suggest that VSMCs express aldosterone synthase, which is up-regulated by phosphate-induced disruption of APEX1-dependent gene suppression. Vascular CYP11B2 may contribute to stimulation of VSMCs osteo-/chondrogenic transformation during hyperphosphatemia.
- Find related publications in this database (using NLM MeSH Indexing)
- Adrenalectomy -
- Aldosterone - biosynthesis
- Aorta - cytology
- Aorta - metabolism
- Coronary Vessels - metabolism
- Cytochrome P-450 CYP11B2 - genetics
- Cytochrome P-450 CYP11B2 - metabolism
- Gene Expression Regulation -
- Humans -
- Mineralocorticoid Receptor Antagonists - pharmacology
- Muscle, Smooth, Vascular - cytology
- Muscle, Smooth, Vascular - metabolism
- Myocytes, Smooth Muscle - cytology
- Myocytes, Smooth Muscle - drug effects
- Myocytes, Smooth Muscle - metabolism
- Osteogenesis - drug effects
- Phosphates - metabolism
- Receptors, Mineralocorticoid - metabolism
- Spironolactone - pharmacology
- Vascular Calcification - metabolism