Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

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Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid Stoffw Microb

Li, C; Rezania, S; Kammerer, S; Sokolowski, A; Devaney, T; Gorischek, A; Jahn, S; Hackl, H; Groschner, K; Windpassinger, C; Malle, E; Bauernhofer, T; Schreibmayer, W.
Piezo1 forms mechanosensitive ion channels in the human MCF-7 breast cancer cell line.
Sci Rep. 2015; 5(2):8364-8364 Doi: 10.1038/srep08364 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG


Führende Autor*innen der Med Uni Graz
Li Chouyang
Schreibmayer Wolfgang
Co-Autor*innen der Med Uni Graz
Bauernhofer Thomas
DeVaney Trevor
Gorischek Astrid
Groschner Klaus
Jahn Stephan
Kammerer Sarah
Malle Ernst
Rezania Simin
Sokolowski Armin
Windpassinger Christian

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Mechanical interaction between cells - specifically distortion of tensional homeostasis-emerged as an important aspect of breast cancer genesis and progression. We investigated the biophysical characteristics of mechanosensitive ion channels (MSCs) in the malignant MCF-7 breast cancer cell line. MSCs turned out to be the most abundant ion channel species and could be activated by negative pressure at the outer side of the cell membrane in a saturable manner. Assessing single channel conductance (GΛ) for different monovalent cations revealed an increase in the succession: Li(+) < Na(+) < K(+) ≈Rb(+) ≈ Cs(+). Divalent cations permeated also with the order: Ca(2+) < Ba(2+). Comparison of biophysical properties enabled us to identify MSCs in MCF-7 as ion channels formed by the Piezo1 protein. Using patch clamp technique no functional MSCs were observed in the benign MCF-10A mammary epithelial cell line. Blocking of MSCs by GsMTx-4 resulted in decreased motility of MCF-7, but not of MCF-10A cells, underscoring a possible role of Piezo1 in invasion and metastatic propagation. The role of Piezo1 in biology and progression of breast cancer is further substantiated by markedly reduced overall survival in patients with increased Piezo1 mRNA levels in the primary tumor.
Find related publications in this database (using NLM MeSH Indexing)
Breast Neoplasms - genetics
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Cell Movement -
Female -
Humans -
Ion Channels - genetics
Ion Channels - metabolism
Ion Transport - genetics
MCF-7 Cells -
Mechanotransduction, Cellular -
Neoplasm Invasiveness -
Neoplasm Proteins - genetics
Neoplasm Proteins - metabolism

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