Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Wutte, N; Archelos, J; Crowe, BA; Zenz, W; Daghofer, E; Fazekas, F; Aberer, E.
Laboratory diagnosis of Lyme neuroborreliosis is influenced by the test used: comparison of two ELISAs, immunoblot and CXCL13 testing.
J Neurol Sci. 2014; 347(1-2):96-103
Doi: 10.1016/j.jns.2014.09.027
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Palfner-Wutte Nora Johanna
- Co-Autor*innen der Med Uni Graz
- Aberer Elisabeth
- Archelos-Garcia Juan-Jose
- Daghofer Elisabeth
- Fazekas Franz
- Zenz Werner
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- Abstract:
- To compare Borrelia-specific intrathecal antibodies by two different ELISAs, an immunoblot (IB) and CXCL13. Twenty-seven adults and 23 children with clinical symptoms compatible with NB were tested for Borrelia-specific intrathecal antibodies by flagellum ELISA-AI (flELISA), a recombinant ELISA-AI (rELISA) and by IB. Patients were classified according to the European Federation of Neurological Societies (EFNS) criteria as definite NB, possible NB, or non-NB. CSF CXCL13 levels were measured by ELISA. Among 50 patients, definite NB was diagnosed with the rELISA-AI in 29 (58%) patients, confirmed by IB in 19/29 patients, with flELISA-AI in 17 (34%) patients, confirmed by IB in 15/17 patients, and with IB in 20 (40%) patients. CXCL13 was positive in 22 (44%) patients. In 4 of 8 patients with negative AI, IB showed many detectable bands both in the CSF and serum. The diagnosis of NB strongly relies on the used test method. The rELISA-AI test appears to be the most sensitive while the flELISA-AI is the least sensitive. However when the ELISA-AIs were confirmed by IB, different patients were identified as NB, while only 26% were identified by all performed test methods. There is a demand for standardized test methods with well-defined sensitivity and specificity to establish validated diagnostic criteria for NB including the use of the IB assay and CXCL13 as an additional non-Borrelia specific determinant in early NB. Copyright © 2014 Elsevier B.V. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Adolescent -
- Adult -
- Aged -
- Borrelia burgdorferi - immunology
- Chemokine CXCL13 - cerebrospinal fluid
- Child -
- Diagnosis, Differential -
- Enzyme-Linked Immunosorbent Assay -
- Female -
- Humans -
- Lyme Neuroborreliosis - cerebrospinal fluid
- Lyme Neuroborreliosis - diagnosis
- Lyme Neuroborreliosis - immunology
- Male -
- Middle Aged -
- Predictive Value of Tests -
- Sensitivity and Specificity -
- Young Adult -
- Find related publications in this database (Keywords)
- CXCL13
- Neuroborreliosis
- Diagnosis
- ELISA
- Immunoblot