Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Balic, M; Schwarzenbacher, D; Stanzer, S; Heitzer, E; Auer, M; Geigl, JB; Cote, RJ; Datar, RH; Dandachi, N.
Genetic and epigenetic analysis of putative breast cancer stem cell models.
BMC Cancer. 2013; 13(7):358-358
Doi: 10.1186/1471-2407-13-358
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Balic Marija
- Dandachi Nadia
- Co-Autor*innen der Med Uni Graz
- Auer Martina
- Geigl Jochen Bernd
- Heitzer Ellen
- Ninaus Daniela
- Stanzer Stefanie
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Cancer stem cell model hypothesizes existence of a small proportion of tumor cells capable of sustaining tumor formation, self-renewal and differentiation. In breast cancer, these cells were found to be associated with CD44⁺CD24-low and ALDH⁺ phenotype. Our study was performed to evaluate the suitability of current approaches for breast cancer stem cell analyses to evaluate heterogeneity of breast cancer cells through their extensive genetic and epigenetic characterization. Breast cancer cell lines MCF7 and SUM159 were cultured in adherent conditions and as mammospheres. Flow cytometry sorting for CD44, CD24 and ALDH was performed. Sorted and unsorted populations, mammospheres and adherent cell cultures were subjected to DNA profiling by array CGH and methylation profiling by Epitect Methyl qPCR array. Methylation status of selected genes was further evaluated by pyrosequencing. Functional impact of methylation was evaluated by mRNA analysis for selected genes. Array CGH did not reveal any genomic differences. In contrast, putative breast cancer stem cells showed altered methylation levels of several genes compared to parental tumor cells. Our results underpin the hypothesis that epigenetic mechanisms seem to play a major role in the regulation of CSCs. However, it is also clear that more efficient methods for CSC enrichment are needed. This work underscores requirement of additional approaches to reveal heterogeneity within breast cancer.
- Find related publications in this database (using NLM MeSH Indexing)
- Antigens, CD24 - analysis
- Breast Neoplasms - genetics
- Cell Line, Tumor -
- Comparative Genomic Hybridization -
- DNA Methylation - genetics
- Epigenesis, Genetic -
- Female -
- Flow Cytometry -
- Gene Expression Regulation, Neoplastic -
- Humans -
- Neoplastic Stem Cells -
- Real-Time Polymerase Chain Reaction -
- Transcriptome -