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SHR Neuro Krebs Kardio Lipid Stoffw Microb

Bach, D; Fuereder, J; Karbiener, M; Scheideler, M; Ress, AL; Neureiter, D; Kemmerling, R; Dietze, O; Wiederstein, M; Berr, F; Plaetzer, K; Kiesslich, T; Pichler, M.
Comprehensive analysis of alterations in the miRNome in response to photodynamic treatment.
J Photochem Photobiol B. 2013; 120(6):74-81 Doi: 10.1016/j.jphotobiol.2013.01.012
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Co-Autor*innen der Med Uni Graz
Karbiener Michael
Lembeck Anna Lena
Pichler Martin

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Photodynamic therapy (PDT) is a local tumour treatment accepted for a number of indications. PDT operates via the cellular stress response through the production of reactive oxygen species and subsequent cellular damage, resulting in cell death. Although PDT-induced signalling and cytotoxicity mechanisms have been investigated, the effect of PDT on microRNA (miRNA) expression is largely unknown. Therefore, we conducted a comprehensive microarray-based analysis of the miRNome of human epidermoid carcinoma cells (A431) following in vitro photodynamic treatment using polyvinylpyrrolidone hypericin (PVPH) as a photosensitiser and nearly homogeneous apoptosis-inducing conditions. Using microarray analysis we found eight miRNAs to be significantly differentially expressed 5h post treatment compared with the baseline levels and three miRNAs with more than 2-fold differential expression that could be detected in 1 or 2 biological replicates. The verification of these results by quantitative RT-PCR including a detailed time-course revealed an up to 15-fold transient over-expression of miR-634, miR-1246, miR-1290 and miR-487b compared with the basal level. For these miRNAs, in silico mRNA target prediction yielded numerous target transcripts involved in the regulation of cell stress, apoptosis, cell adherence and proliferation. This study provides the first comprehensive miRNome analysis after PDT treatment and may help to develop novel miRNA-based therapeutic approaches to further increase the efficiency of PDT. Copyright © 2013 Elsevier B.V. All rights reserved.
Find related publications in this database (using NLM MeSH Indexing)
Cell Line, Tumor -
Humans -
MicroRNAs - genetics
Oligonucleotide Array Sequence Analysis -
Photochemotherapy -
Photosensitizing Agents - pharmacology
Povidone - pharmacology
Time Factors -
Transcriptome - drug effects Transcriptome - radiation effects

Find related publications in this database (Keywords)
Photodynamic therapy
Polyvinylpyrrolidone hypericin
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