Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Patz, S; Trattnig, C; Grünbacher, G; Ebner, B; Gülly, C; Novak, A; Rinner, B; Leitinger, G; Absenger, M; Tomescu, OA; Thallinger, GG; Fasching, U; Wissa, S; Archelos-Garcia, J; Schäfer, U.
More than cell dust: microparticles isolated from cerebrospinal fluid of brain injured patients are messengers carrying mRNAs, miRNAs, and proteins.
J Neurotrauma. 2013; 30(14):1232-1242
Doi: 10.1089/neu.2012.2596
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Patz Silke
- Schäfer Ute
- Co-Autor*innen der Med Uni Graz
- Absenger-Novak Markus
- Archelos-Garcia Juan-Jose
- Gallé Birgit
- Grünbacher Gerda
- Gülly Christian
- Leitinger Gerd
- Maurer Christa
- Novak Alexandra
- Rinner Beate
- Zefferer Ulrike
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Microparticles are cell-derived, membrane-sheathed structures that are believed to shuttle proteins, mRNA, and miRNA to specific local or remote target cells. To date best described in blood, we now show that cerebrospinal fluid (CSF) contains similar structures that can deliver RNAs and proteins to target cells. These are, in particular, molecules associated with neuronal RNA granules and miRNAs known to regulate neuronal processes. Small RNA molecules constituted 50% of the shuttled ribonucleic acid. Using microarray analysis, we identified 81 mature miRNA molecules in CSF microparticles. Microparticles from brain injured patients were more abundant than in non-injured subjects and contained distinct genetic information suggesting that they play a role in the adaptive response to injury. Notably, miR-9 and miR-451 were differentially packed into CSF microparticles derived from patients versus non-injured subjects. We confirmed the transfer of genetic material from CSF microparticles to adult neuronal stem cells in vitro and a subsequent microRNA-specific repression of distinct genes. This first indication of a regulated transport of functional genetic material in human CSF may facilitate the diagnosis and analysis of cerebral modulation in an otherwise inaccessible organ.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- Blotting, Western -
- Brain Injuries - cerebrospinal fluid
- Cell Line -
- Cell-Derived Microparticles - metabolism
- Computational Biology -
- Female -
- Flow Cytometry -
- Gene Silencing -
- Glasgow Coma Scale -
- Humans -
- Immunohistochemistry -
- Male -
- MicroRNAs - metabolism
- Microscopy, Electron -
- Middle Aged -
- Nerve Tissue Proteins - metabolism
- Polymerase Chain Reaction -
- RNA, Messenger - metabolism
- Find related publications in this database (Keywords)
- cerebrospinal fluid
- microparticles
- microRNA
- traumatic brain injury