Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

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Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid Stoffw Microb

Patz, S; Trattnig, C; Grünbacher, G; Ebner, B; Gülly, C; Novak, A; Rinner, B; Leitinger, G; Absenger, M; Tomescu, OA; Thallinger, GG; Fasching, U; Wissa, S; Archelos-Garcia, J; Schäfer, U.
More than cell dust: microparticles isolated from cerebrospinal fluid of brain injured patients are messengers carrying mRNAs, miRNAs, and proteins.
J Neurotrauma. 2013; 30(14):1232-1242 Doi: 10.1089/neu.2012.2596
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Führende Autor*innen der Med Uni Graz
Patz Silke
Schäfer Ute
Co-Autor*innen der Med Uni Graz
Absenger-Novak Markus
Archelos-Garcia Juan-Jose
Gallé Birgit
Grünbacher Gerda
Gülly Christian
Leitinger Gerd
Maurer Christa
Novak Alexandra
Rinner Beate
Zefferer Ulrike

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Scite (citation analytics):

Microparticles are cell-derived, membrane-sheathed structures that are believed to shuttle proteins, mRNA, and miRNA to specific local or remote target cells. To date best described in blood, we now show that cerebrospinal fluid (CSF) contains similar structures that can deliver RNAs and proteins to target cells. These are, in particular, molecules associated with neuronal RNA granules and miRNAs known to regulate neuronal processes. Small RNA molecules constituted 50% of the shuttled ribonucleic acid. Using microarray analysis, we identified 81 mature miRNA molecules in CSF microparticles. Microparticles from brain injured patients were more abundant than in non-injured subjects and contained distinct genetic information suggesting that they play a role in the adaptive response to injury. Notably, miR-9 and miR-451 were differentially packed into CSF microparticles derived from patients versus non-injured subjects. We confirmed the transfer of genetic material from CSF microparticles to adult neuronal stem cells in vitro and a subsequent microRNA-specific repression of distinct genes. This first indication of a regulated transport of functional genetic material in human CSF may facilitate the diagnosis and analysis of cerebral modulation in an otherwise inaccessible organ.
Find related publications in this database (using NLM MeSH Indexing)
Adult -
Aged -
Blotting, Western -
Brain Injuries - cerebrospinal fluid
Cell Line -
Cell-Derived Microparticles - metabolism
Computational Biology -
Female -
Flow Cytometry -
Gene Silencing -
Glasgow Coma Scale -
Humans -
Immunohistochemistry -
Male -
MicroRNAs - metabolism
Microscopy, Electron -
Middle Aged -
Nerve Tissue Proteins - metabolism
Polymerase Chain Reaction -
RNA, Messenger - metabolism

Find related publications in this database (Keywords)
cerebrospinal fluid
traumatic brain injury
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