Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Resch, GP; Goldie, KN; Krebs, A; Hoenger, A; Small, JV.
Visualisation of the actin cytoskeleton by cryo-electron microscopy.
J Cell Sci. 2002; 115(Pt 9):1877-1882
Doi: 10.1242/jcs.115.9.1877
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- Co-Autor*innen der Med Uni Graz
- Krebs Angelika
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- Abstract:
- An understanding of the mechanisms driving cell motility requires clarification of the structural organisation of actin filament arrays in the regions of cell protrusion termed lamellipodia. Currently, there is a lack of consensus on lamellipodia organisation stemming from the application of alternative procedures for ultrastructural visualisation of cytoskeleton networks. In this study, we show that cryo-electron microscopy of extracted cytoskeletons embedded in a thin layer of vitreous ice can reveal the organisation of cytoskeletal elements at high resolution. Since this method involves no dehydration, drying and contrasting steps that can potentially introduce subtle distortions of filament order and interactions, its application opens the way to resolving the controversial details of lamellipodia architecture.
- Find related publications in this database (using NLM MeSH Indexing)
- Actin Cytoskeleton - metabolism
- Actin Cytoskeleton - ultrastructure
- Animals -
- Cell Movement - physiology
- Cryoelectron Microscopy - instrumentation
- Cryoelectron Microscopy - methods
- Eukaryotic Cells - metabolism
- Eukaryotic Cells - ultrastructure
- Membranes, Artificial -
- Mice -
- Pseudopodia - metabolism
- Pseudopodia - ultrastructure
- Tumor Cells, Cultured -
- Find related publications in this database (Keywords)
- actin
- cytoskeleton
- lamellipodium
- motility
- cryo-elecron microscopy