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Gewählte Publikation:

Song, YH; Marx, A; Müller, J; Woehlke, G; Schliwa, M; Krebs, A; Hoenger, A; Mandelkow, E.
Structure of a fast kinesin: implications for ATPase mechanism and interactions with microtubules.
EMBO J. 2001; 20(22):6213-6225 Doi: 10.1093/emboj/20.22.6213 [OPEN ACCESS]
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Krebs Angelika

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We determined the crystal structure of the motor domain of the fast fungal kinesin from Neurospora crassa (NcKin). The structure has several unique features. (i) Loop 11 in the switch 2 region is ordered and enables one to describe the complete nucleotide-binding pocket, including three inter-switch salt bridges between switch 1 and 2. (ii) Loop 9 in the switch 1 region bends outwards, making the nucleotide-binding pocket very wide. The displacement in switch 1 resembles that of the G-protein ras complexed with its guanosine nucleotide exchange factor. (iii) Loop 5 in the entrance to the nucleotide-binding pocket is remarkably long and interacts with the ribose of ATP. (iv) The linker and neck region is not well defined, indicating that it is mobile. (v) Image reconstructions of ice-embedded microtubules decorated with NcKin show that it interacts with several tubulin subunits, including a central beta-tubulin monomer and the two flanking alpha-tubulin monomers within the microtubule protofilament. Comparison of NcKin with other kinesins, myosin and G-proteins suggests that the rate-limiting step of ADP release is accelerated in the fungal kinesin and accounts for the unusually high velocity and ATPase activity.
Find related publications in this database (using NLM MeSH Indexing)
Adenosine Triphosphatases - chemistry
Adenosine Triphosphatases - metabolism
Binding Sites -
Cryoelectron Microscopy -
Crystallography, X-Ray -
DNA-Binding Proteins - chemistry
Dimaprit - analogs & derivatives
Dimaprit - metabolism
GTP-Binding Proteins - chemistry
Image Processing, Computer-Assisted -
Kinesin - chemistry
Kinetics -
Microtubules - metabolism
Models, Molecular -
Myosins - chemistry
Neurospora crassa - chemistry
Nucleotides - metabolism
Protein Binding -
Protein Structure, Tertiary -
Ribose - chemistry
Salts - chemistry
Tubulin - chemistry

Find related publications in this database (Keywords)
motor protein
Neurospora crassa
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