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Krebs, A; Durchschlag, H; Zipper, P.
Small angle X-ray scattering studies and modeling of Eudistylia vancouverii chlorocruorin and Macrobdella decora hemoglobin.
Biophys J. 2004; 87(2):1173-1185 Doi: 10.1529/biophysj.103.037929 [OPEN ACCESS]
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Führende Autor*innen der Med Uni Graz
Krebs Angelika

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Annelids possess giant extracellular oxygen carriers that exhibit a hexagonal bilayer appearance and have molecular masses of approximately 3.5 MDa. By small angle x-ray scattering (SAXS), Eudistylia vancouverii chlorocruorin and Macrobdella decora hemoglobin were investigated in solution. On the basis of the experimental SAXS data, three-dimensional models were established in a two-step approach (trial and error and averaging). The main differences between the complexes concern the structure of their central part and the subunit architecture. Usage of our SAXS models as templates for automated model generation (program DAMMIN) led to refined models that fit perfectly the experimental data. Special attention was paid to the inhomogeneous density distribution observed within the complexes. DAMMIN models without a priori information could not reproducibly locate low-density areas. The usage of templates, however, improved the results considerably, in particular by applying electron microscopy-based templates. Biologically relevant information on the presence of low-density areas and hints for their presumable location could be drawn from SAXS and sophisticated modeling approaches. Provided that different models are analyzed carefully, this obviously opens a way to gain additional biologically relevant structural information from SAXS data.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Annelida - classification
Annelida - metabolism
Computer Simulation -
Hemeproteins - chemistry
Hemeproteins - ultrastructure
Hemoglobins - chemistry
Hemoglobins - ultrastructure
Models, Molecular -
Protein Conformation -
Water - chemistry
X-Ray Diffraction - methods

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