Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

Logo MUG-Forschungsportal

Gewählte Publikation:

Kresse, AE; Million, M; Saperas, E; Taché, Y.
Colitis induces CRF expression in hypothalamic magnocellular neurons and blunts CRF gene response to stress in rats.
Am J Physiol Gastrointest Liver Physiol. 2001; 281(5): G1203-G1213. [OPEN ACCESS]
Web of Science PubMed FullText


Autor/innen der Med Uni Graz:
Kresse Adelheid

Dimensions Citations:

Plum Analytics:
We investigated hypothalamic neuronal corticotropin-releasing factor (CRF) gene expression changes in response to visceral inflammation induced by 2,4,6-trinitrobenzenesulfonic acid (TNB) and acute stress. Seven days after TNB, rats were subjected to water-avoidance stress (WAS) or restraint for 30 min and euthanized. Hypothalamic CRF primary transcripts (heteronuclear RNA, hnRNA) and CRF and arginine vasopressin (AVP) mRNAs were assessed by in situ hybridization. Antisense (35)S-labeled cRNA probes against CRF mRNA intronic and exonic sequences and an oligonucleotide probe against the AVP mRNA were used. TNB induced macroscopic lesions and a fivefold elevation in myeloperoxidase activity in the colon. Colitis increased CRF hnRNA and mRNA signals in the magnocellular part of the paraventricular nucleus of the hypothalamus (PVN) and supraoptic neurons, whereas AVP mRNA was not altered. Colitis did not modify CRF hnRNA signal in the parvocellular part of the PVN (pPVN), plasma corticosterone, and serum osmolarity levels. However, CRF hnRNA expression in the pPVN and the rise in corticosterone and defecation induced by WAS or restraint were blunted in colitic rats. These data show that colitis upregulates CRF gene synthesis in magnocellular hypothalamic neurons but dampens CRF gene transcription in the pPVN and plasma corticosterone responses to environmental acute stressors.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Blood - metabolism
Colitis - chemically induced
Colon - drug effects
Corticosterone - blood
Corticotropin-Releasing Hormone - metabolism
Drinking - drug effects
Eating - drug effects
Gastrointestinal Motility - drug effects
Gene Expression - drug effects
Hypothalamus - metabolism
Male - metabolism
Osmolar Concentration - metabolism
RNA, Heterogeneous Nuclear - metabolism
RNA, Messenger - metabolism
Rats - metabolism
Rats, Sprague-Dawley - metabolism
Stress - genetics
Trinitrobenzenesulfonic Acid - pharmacology

Find related publications in this database (Keywords)
-trinitrobenzenesulfonic acid
arginine vasopressin gene expression
supraoptic nucleus
heteronuclear corticotropin-releasing factor gene expression
food intake
© Med Uni Graz Impressum