Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Bengesser, SA; Reininghaus, EZ; Dalkner, N; Birner, A; Hohenberger, H; Queissner, R; Fellendorf, F; Platzer, M; Pilz, R; Hamm, C; Rieger, A; Kapfhammer, HP; Mangge, H; Reininghaus, B; Meier-Allard, N; Stracke, A; Fuchs, R; Holasek, S.
Endoplasmic reticulum stress in bipolar disorder? - BiP and CHOP gene expression- and XBP1 splicing analysis in peripheral blood.
Psychoneuroendocrinology. 2018; 95(9):113-119
Doi: 10.1016/j.psyneuen.2018.05.029
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Bengesser Susanne
- Reininghaus Eva
- Co-Autor*innen der Med Uni Graz
- Birner Armin
- Dalkner Nina
- Fellendorf Frederike
- Fuchs Robert
- Hamm Carlo
- Holasek Sandra Johanna
- Kapfhammer Hans-Peter
- Mangge Harald
- Meier-Allard Nathalie
- Pilz Rene
- Platzer Martina
- Queissner Robert
- Reininghaus Bernd
- Rieger Alexandra
- Stracke Anika
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- Abstract:
- Endoplasmic Reticulum stress activates the Unfolded Protein Response, which is partially impaired in Bipolar Disorder (BD) according to previous in-vitro studies. Thus, BiP and CHOP gene expression and XBP1 splicing were analyzed in peripheral blood of study participants with BD and controls. RNA was isolated from fasting blood of study participants with BD (n = 81) and controls (n = 54) and reverse transcribed into cDNA. BiP and CHOP gene expression was analyzed with quantitative RT-PCR. Atypical splicing of XBP1 mRNA was measured by semi-quantitative RT-PCR, gel-electrophoresis and densitometry. ANCOVAs with the covariates age, BMI, sex, lithium and anticonvulsants intake were used with SPSS. Bonferroni correction was used to correct for multiple testing (adjusted p = 0.0083). BiP gene expression was significantly higher in BD than in controls (F(1/128) = 10.076, p = 0.002, Partial η2 = 0.073). Total XBP1 (F(1/126) = 9.550, p = 0.002, Partial η2 = 0.070) and unspliced XBP1 (F(1/128)= 8.803, p= 0.004, Patial η2 = 0.065) were significantly decreased in BD. Spliced XBP1 (F(1/126) = 5.848, p = 0.017, Partial η2 = 0.044) and the ratio spliced XBP1/ unspliced XBP1 did not differ between BD and controls (F(1/126) = 0.599, p = 0.441, Partial η2 = 0.005). Gene expression did not differ between euthymia, depression and mania. BiP gene expression was significantly higher in BD compared to controls. Total and unspliced XBP1 were significantly lower in BD than in the control group. Thus, both genes may be considered as putative trait markers. Nevertheless, XBP1 splicing itself did not differ between both groups. Copyright © 2018 Elsevier Ltd. All rights reserved.
- Find related publications in this database (Keywords)
- Bipolar Disorder
- Endoplasmic Reticulum
- ER stress
- Unfolded Protein Response
- BiP
- GRP78
- CHOP
- XBP1