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SHR Neuro Krebs Kardio Lipid Stoffw Microb

Pertl, L; Kern, S; Weger, M; Hausberger, S; Trieb, M; Gasser-Steiner, V; Haas, A; Scharnagl, H; Heinemann, A; Marsche, G.
High-Density Lipoprotein Function in Exudative Age-Related Macular Degeneration.
PLoS One. 2016; 11(5):e0154397-e0154397 Doi: 10.1371/journal.pone.0154397 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG


Führende Autor*innen der Med Uni Graz
Marsche Gunther
Posch-Pertl Laura
Co-Autor*innen der Med Uni Graz
Gasser-Steiner Vanessa
Haas Anton
Heinemann Akos
Pinter-Hausberger Silke
Scharnagl Hubert
Trieb Markus
Weger Martin

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High-density lipoproteins (HDL) have long been implicated in the pathogenesis of age-related macular degeneration (AMD). However, conflicting results have been reported with regard to the associations of AMD with HDL-cholesterol levels. The present study is the first to assess HDL composition and metrics of HDL function in patients with exudative AMD and control patients. Blood samples were collected from 29 patients with exudative AMD and 26 age-matched control patients. Major HDL associated apolipoproteins were determined in apoB-depleted serum by immunoturbidimetry or ELISA, HDL-associated lipids were quantified enzymatically. To get an integrated measure of HDL quantity and quality, we assessed several metrics of HDL function, including cholesterol efflux capacity, anti-oxidative and anti-inflammatory activities using apoB-depleted serum from study participants. In our study, we observed that the HDL associated acute phase protein serum amyloid A (SAA) was significantly increased in AMD patients (p<0.01), whereas all other assessed apolipoproteins including ApoA-I, apoA-II, apoC-II, apoC-III and apoE as well as major HDL associated lipids were not altered. HDL efflux capacity, anti-oxidative capacity and arylesterase activity were not different in AMD patients when compared with the control group. The ability of apoB-depleted serum to inhibit monocyte NF-κB expression was significantly improved in AMD patients (mean difference (MD) -5.6, p<0.01). Moreover, lipoprotein-associated phospholipase A2 activity, a marker of vascular inflammation, was decreased in AMD subjects (MD -24.1, p<0.01). The investigated metrics of HDL composition and HDL function were not associated with exudative AMD in this study, despite an increased content of HDL associated SAA in AMD patients. Unexpectedly, anti-inflammatory activity of apoB-depleted serum was even increased in our study. Our data suggest that the investigated parameters of serum HDL function showed no significant association with exudative AMD. However, we cannot exclude that alterations in locally produced HDL may be part of the AMD pathogenesis.
Find related publications in this database (using NLM MeSH Indexing)
Aged -
Animals -
Anti-Inflammatory Agents - pharmacology
Antioxidants - metabolism
Aryldialkylphosphatase - metabolism
Cholesterol - metabolism
Demography -
Humans -
Lipoproteins, HDL - blood
Mice -
Oxidation-Reduction -
Phospholipases A2 - metabolism
RAW 264.7 Cells -
Serum Amyloid A Protein - metabolism
Wet Macular Degeneration - blood

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