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SHR Neuro Krebs Kardio Lipid

Fickert, P; Trauner, M; Fuchsbichler, A; Zollner, G; Wagner, M; Marschall, HU; Zatloukal, K; Denk, H.
Oncosis represents the main type of cell death in mouse models of cholestasis.
J Hepatol. 2005; 42(3):378-385
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Autor/innen der Med Uni Graz:
Denk Helmut
Fickert Peter
Trauner Michael
Wagner Martin
Zatloukal Kurt
Zollner Gernot
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Abstract:
Since the mechanisms leading to hepatocyte death in cholestasis are not well defined, we aimed to obtain closer insights into the related pathogenetic principles. Cell death was assessed in common bile duct ligated (CBDL) and cholic acid (CA)-fed mice, and compared to Fas agonist Jo2-injected mice by studying H and E-stained tissue sections, DNA ladder analysis, caspase-3-like activity assay, immunohistochemistry, double immunofluorescence microscopy for activated caspase-3 and cytokeratin (CK) 18, the TUNEL method, and electron microscopy. Jo2-treated mice showed activation of caspase-3, breakdown of the CK intermediate filament network, and classical morphological features of apoptosis. In contrast, in CA-fed and CBDL mice, oncosis characterized by cell swelling and ruptured cell membranes was the predominant type of cell death, whereas in both experimental conditions significant activation of caspase-3 was absent and typical CK alterations were rare despite frequent positivity of the TUNEL assay. (i) Oncosis represents the main type of hepatocyte death in acute cholestasis in mice. (ii) The importance of apoptosis in cholestasis may be overestimated if non-specific detection systems (e.g. TUNEL assay) are used.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Apoptosis -
Aspartate Aminotransferases - blood
Bile Acids and Salts - blood
Bile Ducts - physiology
Cell Death -
Cell Membrane - pathology
Cholestasis - pathology
Cholic Acid - toxicity
Disease Models, Animal -
Hepatocytes - pathology Hepatocytes - ultrastructure
Male -
Mice -

Find related publications in this database (Keywords)
apoptosis
bile acids
bile duct ligation
cholestatic liver injury
cholic acid
hepatic artery
necrosis
obstructive jaundice
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