Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Schwarzenbacher, D; Stiegelbauer, V; Deutsch, A; Ress, AL; Aigelsreiter, A; Schauer, S; Wagner, K; Langsenlehner, T; Resel, M; Gerger, A; Ling, H; Ivan, C; Calin, GA; Hoefler, G; Rinner, B; Pichler, M.
Low spinophilin expression enhances aggressive biological behavior of breast cancer.
Oncotarget. 2015; 6(13):11191-11202
Doi: 10.18632/oncotarget.3586
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- Führende Autor*innen der Med Uni Graz
- Ninaus Daniela
- Pichler Martin
- Rinner Beate
- Stiegelbauer Verena
- Co-Autor*innen der Med Uni Graz
- Aigelsreiter Ariane
- Deutsch Alexander
- Gerger Armin
- Höfler Gerald
- Langsenlehner Tanja
- Lembeck Anna Lena
- Resel Margit
- Schauer Silvia
- Wagner Karin
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- Abstract:
- Spinophilin, a putative tumor suppressor gene, has been shown to be involved in the pathogenesis of certain types of cancer, but its role has never been systematically explored in breast cancer. In this study, we determined for the first time the expression pattern of spinophilin in human breast cancer molecular subtypes (n = 489) and correlated it with survival (n = 921). We stably reduced spinophilin expression in breast cancer cells and measured effects on cellular growth, apoptosis, anchorage-independent growth, migration, invasion and self-renewal capacity in vitro and metastases formation in vivo. Microarray profiling was used to determine the most abundantly expressed genes in spinophilin-silenced breast cancer cells. Spinophilin expression was significantly lower in basal-like breast cancer (p<0.001) and an independent poor prognostic factor in breast cancer patients (hazard ratio = 1.93, 95% confidence interval: 1.24 -3.03; p = 0.004) A reduction of spinophilin levels increased cellular growth in breast cancer cells (p<0.05), without influencing activation of apoptosis. Anchorage-independent growth, migration and self-renewal capacity in vitro and metastatic potential in vivo were also significantly increased in spinophilin-silenced cells (p<0.05). Finally, we identified several differentially expressed genes in spinophilin-silenced cells. According to our data, low levels of spinophilin are associated with aggressive behavior of breast cancer.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Apoptosis -
- Biomarkers, Tumor - genetics
- Biomarkers, Tumor - metabolism
- Blotting, Western -
- Breast Neoplasms - genetics
- Breast Neoplasms - metabolism
- Breast Neoplasms - mortality
- Breast Neoplasms - pathology
- Cell Movement -
- Cell Proliferation -
- Cell Transformation, Neoplastic -
- Female -
- Gene Expression Profiling -
- Gene Expression Regulation, Neoplastic -
- Humans -
- Immunoenzyme Techniques -
- Mice -
- Mice, Inbred NOD -
- Mice, SCID -
- Microfilament Proteins - genetics
- Microfilament Proteins - metabolism
- Neoplasm Staging -
- Nerve Tissue Proteins - genetics
- Nerve Tissue Proteins - metabolism
- Prognosis -
- RNA, Messenger - genetics
- Real-Time Polymerase Chain Reaction -
- Reverse Transcriptase Polymerase Chain Reaction -
- Survival Rate -
- Tumor Cells, Cultured -
- Xenograft Model Antitumor Assays -
- Find related publications in this database (Keywords)
- breast cancer
- tumor suppressor
- prognosis
- cellular growth
- invasion