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SHR Neuro Krebs Kardio Lipid Stoffw Microb

Schwab, M; de, Trizio, I; Ghobrial, M; Shiu, JY; Sürücü, O; Girolamo, F; Errede, M; Yilmaz, M; Haybaeck, J; Moiraghi, A; Monnier, PP; Lawler, SE; Greenfield, JP; Radovanovic, I; Frei, K; Schlapbach, R; Vogel, V; Virgintino, D; De, Bock, K; Wälchli, T.
Nucleolin promotes angiogenesis and endothelial metabolism along the onco-fetal axis in the human brain vasculature.
JCI Insight. 2023; Doi: 10.1172/jci.insight.143071
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Co-Autor*innen der Med Uni Graz
Haybäck Johannes

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Glioblastoma is amongst the deadliest human cancers and is highly vascularized. Angiogenesis is very dynamic during brain development, almost quiescent in the adult brain but reactivated in vascular-dependent CNS pathologies including brain tumors. The onco-fetal axis describes the reactivation of fetal programs in tumors, but its relevance in endothelial- and perivascular cells of the human brain vasculature in glial brain tumors is unexplored. Nucleolin is a regulator of cell proliferation and angiogenesis, but its roles in the brain vasculature remain unknown. Here, we studied the expression of Nucleolin in the neurovascular unit in human fetal brains, adult brains and human gliomas in vivo and its effects on sprouting angiogenesis and endothelial metabolism in vitro. Nucleolin is highly expressed in endothelial- and perivascular cells during brain development, downregulated in the adult brain, and upregulated in glioma. Moreover, Nucleolin expression correlated with glioma malignancy in vivo. In culture, siRNA-mediated Nucleolin knock-down reduced human brain endothelial cell (HCMEC) and human umbilical vein endothelial cell (HUVEC) sprouting angiogenesis, proliferation, filopodia extension, and glucose metabolism. Furthermore, inhibition of Nucleolin with the aptamer AS1411 decreased brain endothelial cell proliferation in vitro. Mechanistically, Nucleolin knock-down in HCMECs and HUVECs uncovered regulation of angiogenesis involving VEGFR2 and of endothelial glycolysis. These findings identify Nucleolin as a neurodevelopmental factor reactivated in glioma that promotes sprouting angiogenesis and endothelial metabolism, characterizing Nucleolin as an onco-fetal protein. Our findings have potential implications in the therapeutic targeting of glioma.

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