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Lenard, AJ; Mulder, FAA; Madl, T.
Solvent paramagnetic relaxation enhancement as a versatile method for studying structure and dynamics of biomolecular systems
PROG NUCL MAG RES SP. 2022; 132: 113-139. Doi: 10.1016/j.pnmrs.2022.09.001
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Führende Autor*innen der Med Uni Graz
Lenard Aneta
Madl Tobias
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Abstract:
Solvent paramagnetic relaxation enhancement (sPRE) is a versatile nuclear magnetic resonance (NMR)-based method that allows characterization of the structure and dynamics of biomolecular systems through providing quantitative experimental information on solvent accessibility of NMR-active nuclei. Addition of soluble paramagnetic probes to the solution of a biomolecule leads to paramagnetic relax-ation enhancement in a concentration-dependent manner. Here we review recent progress in the sPRE-based characterization of structural and dynamic properties of biomolecules and their complexes, and aim to deliver a comprehensive illustration of a growing number of applications of the method to various biological systems. We discuss the physical principles of sPRE measurements and provide an overview of available co-solute paramagnetic probes. We then explore how sPRE, in combination with complementary biophysical techniques, can further advance biomolecular structure determination, iden-tification of interaction surfaces within protein complexes, and probing of conformational changes and low-population transient states, as well as deliver insights into weak, nonspecific, and transient interac-tions between proteins and co-solutes. In addition, we present examples of how the incorporation of sol -vent paramagnetic probes can improve the sensitivity of NMR experiments and discuss the prospects of applying sPRE to NMR metabolomics, drug discovery, and the study of intrinsically disordered proteins.(c) 2022 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http:// creativecommons.org/licenses/by/4.0/).

Find related publications in this database (Keywords)
Solvent paramagnetic relaxation
enhancement (sPRE)
Nuclear magnetic resonance (NMR)
spectroscopy
Paramagnetic co-solutes
Structural biology
Solvent accessibility
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