Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

Logo MUG-Forschungsportal

Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid

Koch, C; Kuske, A; Joosse, SA; Yigit, G; Sflomos, G; Thaler, S; Smit, DJ; Werner, S; Borgmann, K; Gartner, S; Mohammadi, PM; Battista, L; Cayrefourcq, L; Altmuller, J; Salinas-Riester, G; Raithatha, K; Zibat, A; Goy, Y; Ott, L; Bartkowiak, K; Tan, TZ; Zhou, Q; Speicher, MR; Muller, V; Gorges, TM; Jucker, M; Thiery, JP; Brisken, C; Riethdorf, S; Alix-Panabieres, C; Pantel, K.
Characterization of circulating breast cancer cells with tumorigenic and metastatic capacity
EMBO MOL MED. 2020; e11908
Web of Science PubMed FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Speicher Michael
Zhou Qing
Altmetrics:

Dimensions Citations:

Plum Analytics:
Abstract:
Functional studies giving insight into the biology of circulating tumor cells (CTCs) remain scarce due to the low frequency ofCTCs and lack of appropriate models. Here, we describe the characterization of a novelCTC-derived breast cancer cell line, designatedCTC-ITB-01, established from a patient with metastatic estrogen receptor-positive (ER+) breast cancer, resistant to endocrine therapy.CTC-ITB-01 remainedER(+)in culture, and copy number alteration (CNA) profiling showed high concordance betweenCTC-ITB-01 andCTCs originally present in the patient with cancer at the time point of blood draw.RNA-sequencing data indicate thatCTC-ITB-01 has a predominantly epithelial expression signature. Primary tumor and metastasis formation in an intraductalPDXmouse model mirrored the clinical progression ofER(+)breast cancer. DownstreamERsignaling was constitutively active inCTC-ITB-01 independent of ligand availability, and theCDK4/6 inhibitor Palbociclib strongly inhibitedCTC-ITB-01 growth. Thus, we established a functional model that opens a new avenue to studyCTCbiology.

Find related publications in this database (Keywords)
breast cancer
circulating tumor cells
functional studies
liquid biopsy
metastasis
© Med Uni Graz Impressum