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SHR Neuro Krebs Kardio Lipid

Grill, M; Hasenoehrl, C; Kienzl, M; Kargl, J; Schicho, R.
Cellular localization and regulation of receptors and enzymes of the endocannabinoid system in intestinal and systemic inflammation.
Histochem Cell Biol. 2019; 151(1):5-20 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Grill Magdalena
Hasenöhrl Carina
Kargl Julia Katharina
Kienzl Melanie
Schicho Rudolf
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Number of Figures: 8
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Abstract:
Surveys suggest that Cannabis provides benefit for people with inflammatory bowel disease. However, mechanisms underlying beneficial effects are not clear. We performed in situ hybridization RNAscope® combined with immunohistochemistry to show cell-specific distribution and regulation of cannabinoid receptor 1 and 2 (CB1, CB2), G protein-coupled receptor 55 (GPR55), and monoacylglycerol lipase (MGL) mRNA in immune cells using murine models of intestinal and systemic inflammation. In healthy animals, the presence in enteric ganglia is high for CB1 mRNA, but low for CB2 and GPR55 mRNAs. MGL mRNA is predominant throughout the intestinal wall including myenteric neurons, epithelium, circular and longitudinal muscular layers, and the lamina propria. Within the immune system, B220+ cells exhibit high gene expression for CB2 while the expression of CB2 in F4/80+ and CD3+ cells is less prominent. In contrast, GPR55 mRNA is highly present in F4/80+ and CD3+ cells. qRT-PCR of total colonic segments shows that the expression of GPR55 and MGL genes drops during intestinal inflammation. Also at cellular levels, GPR55 and MGL gene expression is reduced in F4/80+, but not CD3+ cells. As to systemic inflammation, reduced gene expression of MGL is observed in ileum by qRT-PCR, while at cellular levels, altered gene expression is also seen for CB1 and GPR55 in CD3+ but not F4/80+ cells. In summary, our study reveals changes in gene expression of members of the endocannabinoid system in situ attesting particularly GPR55 and MGL a distinct cellular role in the regulation of the immune response to intestinal and systemic inflammation.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Asialoglycoproteins - analysis
Asialoglycoproteins - deficiency
Asialoglycoproteins - metabolism
Dextran Sulfate -
Endocannabinoids - metabolism
Immunohistochemistry -
In Situ Hybridization -
Inflammation - chemically induced
Inflammation - metabolism
Inflammation - pathology
Intestines - chemistry
Intestines - pathology
Lectins, C-Type - analysis
Lectins, C-Type - deficiency
Lectins, C-Type - metabolism
Lipopolysaccharides -
Membrane Proteins - analysis
Membrane Proteins - deficiency
Membrane Proteins - metabolism
Mice -
Mice, Inbred C57BL -
Mice, Knockout -
RNA, Messenger - analysis
RNA, Messenger - genetics
RNA, Messenger - metabolism
Receptor, Cannabinoid, CB1 - analysis
Receptor, Cannabinoid, CB1 - deficiency
Receptor, Cannabinoid, CB1 - metabolism
Receptor, Cannabinoid, CB2 - analysis
Receptor, Cannabinoid, CB2 - deficiency
Receptor, Cannabinoid, CB2 - metabolism
Receptors, Cannabinoid - analysis
Receptors, Cannabinoid - metabolism

Find related publications in this database (Keywords)
Cannabinoid receptors
GPR55
CB1
CB2
MGL
In situ hybridization
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