Gewählte Publikation:
SHR
Neuro
Krebs
Kardio
Lipid
Eroglu, E; Charoensin, S; Bischof, H; Ramadani, J; Gottschalk, B; Depaoli, MR; Waldeck-Weiermair, M; Graier, WF; Malli, R.
Genetic biosensors for imaging nitric oxide in single cells.
Free Radic Biol Med. 2018;
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Autor/innen der Med Uni Graz:
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Bischof Helmut
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Depaoli Maria Rosa
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Eroglu Emrah
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Gottschalk Benjamin
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Graier Wolfgang
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Malli Roland
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Ramadani-Muja Jeta
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Waldeck-Weiermair Markus
- Altmetrics:
- Dimensions Citations:
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- Number of Figures: 7
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- Abstract:
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Over the last decades a broad collection of sophisticated fluorescent protein-based probes was engineered with the aim to specifically monitor nitric oxide (NO), one of the most important signaling molecules in biology. Here we report and discuss the characteristics and fields of applications of currently available genetically encoded fluorescent sensors for the detection of NO and its metabolites in different cell types.
Because of its radical nature and short half-life, real-time imaging of NO on the level of single cells is challenging. Herein we review state-of-the-art genetically encoded fluorescent sensors for NO and its byproducts such as peroxynitrite, nitrite and nitrate. Such probes enable the real-time visualization of NO signals directly or indirectly on the level of single cells and cellular organelles and, hence, extend our understanding of the spatiotemporal dynamics of NO formation, diffusion and degradation. Here, we discuss the significance of NO detection in individual cells and on subcellular level with genetic biosensors. Currently available genetically encoded fluorescent probes for NO and nitrogen species are critically discussed in order to provide insights in the functionality and applicability of these promising tools. As an outlook we provide ideas for novel approaches for the design and application of improved NO probes and fluorescence imaging protocols.
Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.
- Find related publications in this database (Keywords)
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Nitric oxide
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Genetically encoded fluorescent probes
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FRET
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Fluorescence microscopy
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Single cell imaging