Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

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Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid

Bischof, H; Rehberg, M; Stryeck, S; Artinger, K; Eroglu, E; Waldeck-Weiermair, M; Gottschalk, B; Rost, R; Deak, AT; Niedrist, T; Vujic, N; Lindermuth, H; Prassl, R; Pelzmann, B; Groschner, K; Kratky, D; Eller, K; Rosenkranz, AR; Madl, T; Plesnila, N; Graier, WF; Malli, R.
Novel genetically encoded fluorescent probes enable real-time detection of potassium in vitro and in vivo.
Nat Commun. 2017; 8(1): 1422-1422. [OPEN ACCESS]
PubMed PUBMED Central FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Artinger Katharina
Bischof Helmut
Deak Andras Tamas
Eller Kathrin
Eroglu Emrah
Gottschalk Benjamin
Graier Wolfgang
Groschner Klaus
Kratky Dagmar
Madl Tobias
Malli Roland
Niedrist Tobias
Pelzmann Brigitte
Prassl Ruth
Rosenkranz Alexander
Rost René
Stryeck Sarah
Vujic Nemanja
Waldeck-Weiermair Markus
Gendermonitor:
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Number of Figures: 6
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Abstract:
Changes in intra- and extracellular potassium ion (K(+)) concentrations control many important cellular processes and related biological functions. However, our current understanding of the spatiotemporal patterns of physiological and pathological K(+) changes is severely limited by the lack of practicable detection methods. We developed K(+)-sensitive genetically encoded, Förster resonance energy transfer-(FRET) based probes, called GEPIIs, which enable quantitative real-time imaging of K(+) dynamics. GEPIIs as purified biosensors are suitable to directly and precisely quantify K(+) levels in different body fluids and cell growth media. GEPIIs expressed in cells enable time-lapse and real-time recordings of global and local intracellular K(+) signals. Hitherto unknown Ca(2+)-triggered, organelle-specific K(+) changes were detected in pancreatic beta cells. Recombinant GEPIIs also enabled visualization of extracellular K(+) fluctuations in vivo with 2-photon microscopy. Therefore, GEPIIs are relevant for diverse K(+) assays and open new avenues for live-cell K(+) imaging.

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