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SHR Neuro Krebs Kardio Lipid Stoffw Microb

Weiss, B; Pichler, A; Damulina, A; Buchmann, A; Hochmeister, S; Seifert-Held, T; Enzinger, C; Archelos, JJ; Khalil, M.
Evidence for an Intrathecal Immunoglobulin Synthesis by Kappa Free Light Chains in Neurological Patients with an Isolated Band in Isoelectric Focusing.
Biomedicines. 2022; 10(9): Doi: 10.3390/biomedicines10092202 [OPEN ACCESS]
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Führende Autor*innen der Med Uni Graz

Khalil Michael

Weiß Bastian

Co-Autor*innen der Med Uni Graz

Archelos-Garcia Juan-Jose

Buchmann Arabella

Damulina Anna

Enzinger Christian

Hochmeister Sonja

Pichler Alexander

Seifert-Held Thomas

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Abstract:

The gold standard for detecting intrathecal immunoglobulin synthesis is the determination of the oligoclonal band (OCB) in the cerebrospinal fluid (CSF) using isoelectric focusing (IEF). Controversy still exists regarding the significance of an isolated band in the CSF. A highly promising alternative method for the assessment of intrathecal inflammation is the quantification of kappa free light chains (k-FLC). Our aim was to evaluate the clinical significance of quantitative k-FLC in patients with an isolated band in the CSF. Using the Human Kappa Freelite Mx Kit on a turbidimetric Optilite^®, we quantified the k-FLCs in paired CSF and serum samples in 47 patients with a single band in IEF. We classified patients into 27× inflammatory neurological disorders (IND), 2× peripheral inflammatory neurological disorders (PIND), 9× non-inflammatory neurological disorders (NIND) and 9× symptomatic controls (SC) based on their medical diagnosis. k-FLC were below the lower measurement limit of the analyser (LML) in all SC and PIND, as well as in 8 out of 9 NIND and 11 IND. Only 1 NIND and 16 IND were above the LML, and of these, only 14 IND were above the upper discrimination limit (Qlim). A neuroinflammatory nature of the diseases can be indicated in many cases by positive k-FLC in patients with an isolated band in IEF. The measurement of k-FLC can support the diagnosis of neurological diseases if they are included in the routine work-up.

Find related publications in this database (Keywords)

immunoglobulin kappa-chains

isoelectric focusing

neuroinflammatory diseases

multiple sclerosis

human

biomarker

cerebrospinal fluid

central nervous system

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