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SHR Neuro Krebs Kardio Lipid Stoffw Microb

Valadez-Cosmes, P; Maitz, K; Kindler, O; Raftopoulou, S; Kienzl, M; Santiso, A; Mihalic, ZN; Brcic, L; Lindenmann, J; Fediuk, M; Pichler, M; Schicho, R; Houghton, AM; Heinemann, A; Kargl, J.
Identification of Novel Low-Density Neutrophil Markers Through Unbiased High-Dimensional Flow Cytometry Screening in Non-Small Cell Lung Cancer Patients.
Front Immunol. 2021; 12: 703846 Doi: 10.3389/fimmu.2021.703846 [OPEN ACCESS]
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Führende Autor*innen der Med Uni Graz

Kargl Julia

Valadez Cosmes Paulina

Co-Autor*innen der Med Uni Graz

Brcic Luka

Fediuk Melanie

Heinemann Akos

Kienzl Melanie

Kindler Oliver

Lindenmann Jörg

Maitz Kathrin

Mihalic Zala Nikita

Pichler Martin

Raftopoulou Sofia

Santiso Sanchez Ana Luisa

Schicho Rudolf

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Abstract:

Neutrophils have been described as a phenotypically heterogeneous cell type that possess both pro- and anti-tumor properties. Recently, a subset of neutrophils isolated from the peripheral blood mononuclear cell (PBMC) fraction has been described in cancer patients. These low-density neutrophils (LDNs) show a heterogeneous maturation state and have been associated with pro-tumor properties in comparison to mature, high-density neutrophils (HDNs). However, additional studies are necessary to characterize this cell population. Here we show new surface markers that allow us to discriminate between LDNs and HDNs in non-small cell lung cancer (NSCLC) patients and assess their potential as diagnostic/prognostic tool. LDNs were highly enriched in NSCLC patients (median=20.4%, range 0.3-76.1%; n=26) but not in healthy individuals (median=0.3%, range 0.1-3.9%; n=14). Using a high-dimensional human cell surface marker screen, we identified 12 surface markers that were downregulated in LDNs when compared to HDNs, while 41 surface markers were upregulated in the LDN subset. Using flow cytometry, we confirmed overexpression of CD36, CD41, CD61 and CD226 in the LDN fraction. In summary, our data support the notion that LDNs are a unique neutrophil population and provide novel targets to clarify their role in tumor progression and their potential as diagnostic and therapeutic tool.

Find related publications in this database (Keywords)

low-density neutrophils

non-small cell lung cancer (NSCLC)

high-dimensional flow cytometry

CD36

CD41

CD61

CD226

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