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SHR Neuro Krebs Kardio Lipid Stoffw Microb

Benezeder, T; Tiran, V; Treitler, AAN; Suppan, C; Rossmann, C; Stoeger, H; Cote, RJ; Datar, RH; Balic, M; Dandachi, N.
Multigene methylation analysis of enriched circulating tumor cells associates with poor progression-free survival in metastatic breast cancer patients.
Oncotarget. 2017; 8(54):92483-92496 Doi: 10.18632/oncotarget.21426 [OPEN ACCESS]
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Führende Autor*innen der Med Uni Graz

Balic Marija

Benezeder Theresa Helena

Dandachi Nadia

Co-Autor*innen der Med Uni Graz

Roßmann Christopher Herbert

Stöger Herbert

Suppan Christoph

Tiran Verena

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Abstract:

Blood-based biomarkers such as circulating tumor cells (CTCs) provide dynamic real-time assessment of molecular tumor characteristics beyond the primary tumor. The aim of this study was to evaluate the feasibility of a size-based microfilter to assess multigene methylation analysis of enriched CTCs in a prospective proof-of principle study. We examined the quantitative methylation status of nine genes (AKR1B1, BMP6, CST6, HOXB4, HIST1H3C, ITIH5, NEUROD1, RASSF1, SOX17) in enriched CTCs from metastatic breast cancer patients. Feasibility and clinical performance testing were assessed in a test set consisting of 37 patients and 25 healthy controls. With established cut-off values from the healthy control group, methylation of enriched CTCs was detected in at least one gene in 18/37 patients (48.6%), while 97.8% of all control samples were unmethylated. Patients with CTCs unmethylated for CST6, ITIH5, or RASSF1 showed significantly longer PFS compared to patients with corresponding enriched methylated CTCs. This proof-of-principle study shows the feasibility of a size-based microfilter to enrich and analyze multigene methylation profile of CTCs from metastatic breast cancer patients. For the first time, we report that multigene methylation analysis of enriched CTCs provides prognostic information in metastatic breast cancer patients.

Find related publications in this database (Keywords)

circulating tumor cells

enrichment

metastatic breast cancer

methylation

prognosis

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