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Birkl, C; Langkammer, C; Golob-Schwarzl, N; Leoni, M; Haybaeck, J; Goessler, W; Fazekas, F; Ropele, S.
Effects of formalin fixation and temperature on MR relaxation times in the human brain.
NMR Biomed. 2016; 29(4):458-465 Doi: 10.1002/nbm.3477
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Führende Autor*innen der Med Uni Graz

Birkl Christoph

Ropele Stefan

Co-Autor*innen der Med Uni Graz

Fazekas Franz

Golob-Schwarzl Nicole

Haybäck Johannes

Langkammer Christian

Leoni Marlene

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Abstract:

Post-mortem MRI of the brain is increasingly applied in neuroscience for a better understanding of the contrast mechanisms of disease induced tissue changes. However, the influence of chemical processes caused by formalin fixation and differences in temperature may hamper the comparability with results from in vivo MRI. In this study we investigated how formalin fixation and temperature affect T1, T2 and T2* relaxation times of brain tissue. Fixation effects were examined with respect to changes in water content and crosslinking. Relaxometry was performed in brain slices from five deceased subjects at different temperatures. All measurements were repeated after 190 days of formaldehyde immersion. The water content of unfixed and fixed tissue was determined using the wet-to-dry ratio following drying. Protein weight was determined with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Fixation caused a strong decrease of all relaxation times, the strongest effect being seen on T1, with a reduction of up to 76%. The temperature coefficient of T1 was lower in the fixed than unfixed tissue, which was in contrast to T2, where an increase of the temperature coefficient was observed following fixation. The reduction of the water content after fixation was in the range of 1-6% and thus not sufficient to explain the changes in relaxation time. Results from SDS-PAGE indicated a strong increase of the protein size above 260 kDa in all brain structures examined. Our results suggest that crosslinking induced changes of the macromolecular matrix are responsible for T1 shortening and a decreased temperature dependency. The relaxation times provided in this work should allow optimization of post-mortem MRI protocols for the brain. Copyright © 2016 John Wiley & Sons, Ltd.

Find related publications in this database (Keywords)

MRI

relaxation times

formalin fixation

human brain

temperature

post-mortem MRI

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