Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid Stoffw Microb

Berger, M; Thueringer, A; Franz, D; Dandachi, N; Talakić, E; Richtig, G; Richtig, E; Rohrer, PM; Koch, L; Wolf, IH; Koch, C; Rainer, BM; Koeller, M; Pichler, M; Gerritsmann, H; Kashofer, K; Aigelsreiter, A.
Circulating Tumor DNA as a Marker for Treatment Response in Metastatic Melanoma Patients Using Next-Generation Sequencing-A Prospective Feasibility Study.
Cancers (Basel). 2021; 13(12): Doi: 10.3390/cancers13123101 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG


Führende Autor*innen der Med Uni Graz
Aigelsreiter Ariane
Berger Marina
Kashofer Karl
Co-Autor*innen der Med Uni Graz
Dandachi Nadia
Franz Doritt Franziska
Koch Catharina
Koch Lukas
Pichler Martin
Rainer Barbara
Richtig Erika
Richtig Georg
Rohrer Peter Michael
Talakic Emina
Wolf Ingrid

Dimensions Citations:

Plum Analytics:

Scite (citation analytics):

We prospectively performed a longitudinal analysis of circulating tumor DNA (ctDNA) from 149 plasma samples and CT scans in Stage III and IV metastatic melanoma patients (n = 20) treated with targeted agents or immunotherapy using two custom next-generation sequencing (NGS) Ion AmpliSeq™ HD panels including 60 and 81 amplicons in 18 genes, respectively. Concordance of matching cancer-associated mutations in tissue and plasma was 73.3%. Mutant allele frequency (MAF) levels showed a range from 0.04% to 28.7%, well detectable with NGS technologies utilizing single molecule tagging like the AmpliSeq™ HD workflow. Median followup time of the tissue and/or plasma positive cohort (n = 15) was 24.6 months and median progression-free survival (PFS) was 7.8 months. Higher MAF ≥ 1% at baseline was not significantly associated with a risk of progression (Odds Ratio = 0.15; p = 0.155). Although a trend could be seen, MAF levels did not differ significantly over time between patients with and without a PFS event (p = 0.745). Depending on the cell-free DNA amount, NGS achieved a sensitivity down to 0.1% MAF and allowed for parallel analysis of multiple mutations and previously unknown mutations. Our study indicates that NGS gene panels could be useful for monitoring disease burden during therapy with ctDNA in melanoma patients.

Find related publications in this database (Keywords)
metastatic melanoma
circulating tumor DNA
next-generation sequencing
custom panels
treatment monitoring
© Med Uni Graz Impressum