Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid Stoffw Microb

Li, C; Rezania, S; Kammerer, S; Sokolowski, A; Devaney, T; Gorischek, A; Jahn, S; Hackl, H; Groschner, K; Windpassinger, C; Malle, E; Bauernhofer, T; Schreibmayer, W.
Piezo1 forms mechanosensitive ion channels in the human MCF-7 breast cancer cell line.
Sci Rep. 2015; 5(2):8364-8364 Doi: 10.1038/srep08364 [OPEN ACCESS]
Web of Science PubMed FullText FullText_MUG

 

Führende Autor*innen der Med Uni Graz

Li Chouyang

Schreibmayer Wolfgang

Co-Autor*innen der Med Uni Graz

Bauernhofer Thomas

DeVaney Trevor

Gorischek Astrid

Groschner Klaus

Jahn Stephan

Kammerer Sarah

Malle Ernst

Rezania Simin

Sokolowski Armin

Windpassinger Christian

Altmetrics:

Dimensions Citations:

Plum Analytics:

Scite (citation analytics):

Abstract:

Mechanical interaction between cells - specifically distortion of tensional homeostasis-emerged as an important aspect of breast cancer genesis and progression. We investigated the biophysical characteristics of mechanosensitive ion channels (MSCs) in the malignant MCF-7 breast cancer cell line. MSCs turned out to be the most abundant ion channel species and could be activated by negative pressure at the outer side of the cell membrane in a saturable manner. Assessing single channel conductance (GΛ) for different monovalent cations revealed an increase in the succession: Li(+) < Na(+) < K(+) ≈Rb(+) ≈ Cs(+). Divalent cations permeated also with the order: Ca(2+) < Ba(2+). Comparison of biophysical properties enabled us to identify MSCs in MCF-7 as ion channels formed by the Piezo1 protein. Using patch clamp technique no functional MSCs were observed in the benign MCF-10A mammary epithelial cell line. Blocking of MSCs by GsMTx-4 resulted in decreased motility of MCF-7, but not of MCF-10A cells, underscoring a possible role of Piezo1 in invasion and metastatic propagation. The role of Piezo1 in biology and progression of breast cancer is further substantiated by markedly reduced overall survival in patients with increased Piezo1 mRNA levels in the primary tumor.

Find related publications in this database (using NLM MeSH Indexing)

Breast Neoplasms - genetics

Breast Neoplasms - metabolism

Breast Neoplasms - pathology

Cell Movement -

Female -

Humans -

Ion Channels - genetics

Ion Channels - metabolism

Ion Transport - genetics

MCF-7 Cells -

Mechanotransduction, Cellular -

Neoplasm Invasiveness -

Neoplasm Proteins - genetics

Neoplasm Proteins - metabolism

© Med Uni Graz • Impressum