Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid Stoffw Microb

Stiegler, P; Sereinigg, M; Puntschart, A; Bradatsch, A; Seifert-Held, T; Wiederstein-Grasser, I; Leber, B; Stadelmeyer, E; Dandachi, N; Zelzer, S; Iberer, F; Stadlbauer, V.
Oxidative stress and apoptosis in a pig model of brain death (BD) and living donation (LD).
J Transl Med. 2013; 11(10):244-244 Doi: 10.1186/1479-5876-11-244 [OPEN ACCESS]
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Führende Autor*innen der Med Uni Graz
Stiegler Philipp
Co-Autor*innen der Med Uni Graz
Dandachi Nadia
Iberer Florian
Leber Bettina
Puntschart Andreas Jürgen
Seifert-Held Thomas
Sereinigg Michael
Simon Andrea
Stadelmeyer Elke
Stadlbauer-Köllner Vanessa
Wiederstein-Grasser Iris
Zelzer Sieglinde

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As organ shortage is increasing, the acceptance of marginal donors increases, which might result in poor organ function and patient survival. Mostly, organ damage is caused during brain death (BD), cold ischemic time (CIT) or after reperfusion due to oxidative stress or the induction of apoptosis. The aim of this study was to study a panel of genes involved in oxidative stress and apoptosis and compare these findings with immunohistochemistry from a BD and living donation (LD) pig model and after cold ischemia time (CIT). BD was induced in pigs; after 12 h organ retrieval was performed; heart, liver and kidney tissue specimens were collected in the BD (n = 6) and in a LD model (n = 6). PCR analysis for NFKB1, GSS, SOD2, PPAR-alpha, OXSR1, BAX, BCL2L1, and HSP 70.2 was performed and immunohistochemistry used to show apoptosis and nitrosative stress induced cell damage. In heart tissue of BD BAX, BCL2L1 and HSP 70.2 increased significantly after CIT. Only SOD2 was over-expressed after CIT in BD liver tissue. In kidney tissue, BCL2L1, NFKB, OXSR1, SOD2 and HSP 70.2 expression was significantly elevated in LD. Immunohistochemistry showed a significant increase in activated Caspase 3 and nitrotyrosine positive cells after CIT in BD in liver and in kidney tissue but not in heart tissue. The up-regulation of protective and apoptotic genes seems to be divergent in the different organs in the BD and LD setting; however, immunohistochemistry revealed more apoptotic and nitrotyrosine positive cells in the BD setting in liver and kidney tissue whereas in heart tissue both BD and LD showed an increase.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Apoptosis - genetics
Brain Death - pathology
Caspase 3 - metabolism
Disease Models, Animal -
Gene Expression Regulation -
Immunohistochemistry -
Kidney - metabolism
Liver - metabolism
Mice -
Myocardium - metabolism
Oxidative Stress - genetics
Polymerase Chain Reaction -
Sus scrofa -

Find related publications in this database (Keywords)
Organ donation
Brain death
Living donation
Oxidative stress
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